Drosophila Transgenic Laboratory, Section of Genetics, Department of Zoology, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, Uttar Pradesh, India.
Food Chem Toxicol. 2013 May;55:29-35. doi: 10.1016/j.fct.2012.12.028. Epub 2013 Jan 11.
The role of Eucalyptus citriodora L. leaf extract was studied on the transgenic Drosophila model of flies expressing normal human alpha synuclein (h-αS) in the neurons. These flies exhibit locomotor dysfunction as the age progresses. The leaf extract was prepared in acetone and was subjected to GC-MS analysis. The GC-MS analysis revealed the presence of nine major compounds. E. citriodora extract at final concentration of 0.25, 0.50 and 1.0μl/ml was supplemented with the diet and the flies were allowed to feed for 21days. The effect of extract was studied on the climbing ability and the oxidative stress on the PD model Drosophila expressing normal human alpha synuclein (h-αS) in the neurons. The supplementation of 0.25, 0.50 and 1.0μl/ml of E. citriodora extract showed a dose dependent significant delay in the loss of climbing ability and reduction in the oxidative stress in the brain of PD model flies. The results also support the utility of this model in studying PD symptoms.
研究了柠檬桉叶提取物在表达正常人类α-突触核蛋白（h-αS）的神经元的转基因果蝇模型中的作用。这些果蝇随着年龄的增长表现出运动功能障碍。将叶提取物用丙酮制备，并进行 GC-MS 分析。GC-MS 分析显示存在九种主要化合物。将浓度为 0.25、0.50 和 1.0μl/ml 的柠檬桉提取物添加到饮食中，并允许果蝇进食 21 天。研究了提取物对表达正常人类α-突触核蛋白（h-αS）的神经元的 PD 模型果蝇的攀爬能力和氧化应激的影响。补充 0.25、0.50 和 1.0μl/ml 的柠檬桉提取物显示出剂量依赖性的显著延迟，可减缓 PD 模型果蝇失去攀爬能力和减少大脑中的氧化应激。该结果还支持了该模型在研究 PD 症状中的应用。
