Drosophila Transgenic Laboratory, Section of Genetics, Department of Zoology, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, Uttar Pradesh 202002, India.
Photobiology Division, CSIR-Indian Institute of Toxicology Research, Lucknow, Uttar Pradesh 206002, India.
Parkinsons Dis. 2014;2014:262058. doi: 10.1155/2014/262058. Epub 2014 Nov 27.
The role of Centella asiatica L. leaf extract was studied on the transgenic Drosophila model flies expressing normal human alpha synuclein (h-αS) in the neurons. The leaf extract was prepared in acetone and was subjected to GC-MS analysis. C. asiatica extract at final concentration of 0.25, 0.50, and 1.0 μL/mL was mixed with the diet and the flies were allowed feeding on it for 24 days. The effect of extract was studied on the climbing ability, activity pattern, lipid peroxidation, protein carbonyl content, glutathione content, and glutathione-S-transferase activity in the brains of transgenic Drosophila. The exposure of extract to PD model flies results in a significant delay in the loss of climbing ability and activity pattern and reduced the oxidative stress (P < 0.05) in the brains of PD flies as compared to untreated PD flies. The results suggest that C. asiatica leaf extract is potent in reducing the PD symptoms in transgenic Drosophila model of Parkinson's disease.
积雪草叶提取物在表达正常人类α-突触核蛋白（h-αS）的神经元中的转基因果蝇模型中的作用进行了研究。将叶提取物用丙酮制备，并进行 GC-MS 分析。积雪草提取物终浓度为 0.25、0.50 和 1.0 μL/mL 与饮食混合，让果蝇在其上进食 24 天。研究了提取物对转基因果蝇大脑的攀爬能力、活动模式、脂质过氧化、蛋白质羰基含量、谷胱甘肽含量和谷胱甘肽-S-转移酶活性的影响。与未经处理的 PD 果蝇相比，提取物暴露于 PD 模型果蝇会导致其失去攀爬能力和活动模式的显著延迟，并且降低了 PD 果蝇大脑中的氧化应激（P < 0.05）。结果表明，积雪草叶提取物在减少帕金森病转基因果蝇模型的 PD 症状方面具有潜力。
