Departments of Pediatrics and Obstetrics and Gynecology, Louisiana State University Health Sciences Center, Shreveport, LA 71130, USA.
Neonatology. 2012;102(3):163-8. doi: 10.1159/000339286. Epub 2012 Jul 4.
Infants of diabetic mothers (IDMs) are at increased risk for metabolic complications. Type 1 and some type 2 diabetic patients have elevated levels of the ketone bodies acetoacetate (AA) and β-hydroxybutyrate (BHB).
The aim of this study was to examine how hyperketonemia in diabetic mothers affects markers of inflammation and oxidative stress in their offspring.
Blood was obtained from 23 diabetic mothers and 13 healthy mothers and their infants' umbilical cords at delivery. Interleukin-8, monocyte chemotactic protein-1 (MCP-1) and protein carbonyl (protein oxidation) levels were determined by ELISA. U937 human monocyte cell culture was used to examine the effect of AA and BHB on secretion of MCP-1.
There was a significant increase in the levels of AA in cord blood of IDMs compared with cord blood of infants of healthy mothers. A significant increase in the levels of protein oxidation (p < 0.05) and MCP-1 levels (p < 0.05) was observed in the cord blood of IDMs. The level of MCP-1 correlated significantly (r = 0.51, p = 0.01) with the concentration of AA in the IDMs. In further experiments with cultured monocytes treated with exogenous AA (0-4 mM), a significant increase in MCP-1 secretion was observed in AA- but not BHB-treated monocytes.
Blood levels of AA and MCP-1 are elevated in IDMs, which may contribute to the development of the metabolic complications seen in IDMs.
糖尿病母亲所生婴儿（IDM）代谢并发症风险增加。1 型和部分 2 型糖尿病患者的酮体乙酰乙酸（AA）和β-羟丁酸（BHB）水平升高。
本研究旨在研究糖尿病母亲的高酮血症如何影响其后代的炎症和氧化应激标志物。
分娩时从 23 名糖尿病母亲和 13 名健康母亲及其婴儿的脐血中采集血液。通过 ELISA 测定白细胞介素-8（IL-8）、单核细胞趋化蛋白-1（MCP-1）和蛋白羰基（蛋白氧化）水平。使用 U937 人单核细胞培养物来研究 AA 和 BHB 对 MCP-1 分泌的影响。
与健康母亲婴儿的脐血相比，IDM 的脐血中 AA 水平显著升高。IDM 的脐血中蛋白氧化（p<0.05）和 MCP-1 水平（p<0.05）显著增加。IDM 中 MCP-1 水平与 AA 浓度显著相关（r=0.51，p=0.01）。在进一步用外源性 AA（0-4 mM）处理培养的单核细胞的实验中，AA 处理的单核细胞中 MCP-1 分泌显著增加，但 BHB 处理的单核细胞中无此变化。
IDM 的血液 AA 和 MCP-1 水平升高，这可能导致 IDM 中代谢并发症的发生。
