Que Youxiong, Xu Liping, Lin Jianwei, Luo Jun, Xu Jingsheng, Zheng Jin, Chen Rukai
Key Lab of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China.
J Biomed Biotechnol. 2012;2012:390107. doi: 10.1155/2012/390107. Epub 2012 Jun 19.
Erianthus arundinaceum is a wild relative species of sugarcane. The aim of this research was to demonstrate the feasibility of cDNA-SRAP for differential gene expression and to explore the molecular mechanism of drought resistance in E. arundinaceum. cDNA-SRAP technique, for the first time, was applied in the analysis of differential gene expression in E. arundinaceum under drought stress. In total, eight differentially expressed genes with length of 185-427 bp were successfully isolated (GenBank Accession numbers: EU071770, EU071772, EU071774, EU071776, EU071777, EU071779, EU071780, and EU071781). Based on their homologies with genes in GenBank, these genes were assumed to encode ribonuclease III, vacuolar protein, ethylene insensitive protein, aerobactin biosynthesis protein, photosystem II protein, glucose transporter, leucine-rich repeat protein, and ammonia monooxygenase. Real-time PCR analysis on the expression profiling of gene (EU071774) encoding ethylene-insensitive protein and gene (EU071781) encoding ammonia monooxygenase revealed that the expression of these two genes was upregulated both by PEG and ABA treatments, suggesting that they may involve in the drought resistance of E. arundinaceum. This study constitutes the first report of genes activated in E. arundinaceum by drought stress and opens up the application of cDNA-SRAP in differential gene expression analysis in E. arundinaceum under certain stress conditions.
斑茅是甘蔗的野生近缘种。本研究的目的是证明cDNA-SRAP用于差异基因表达分析的可行性，并探索斑茅抗旱的分子机制。cDNA-SRAP技术首次应用于分析干旱胁迫下斑茅的差异基因表达。总共成功分离出8个长度为185 - 427 bp的差异表达基因（GenBank登录号：EU071770、EU071772、EU071774、EU071776、EU071777、EU071779、EU071780和EU071781）。根据它们与GenBank中基因的同源性，推测这些基因编码核糖核酸酶III、液泡蛋白、乙烯不敏感蛋白、气杆菌素生物合成蛋白、光系统II蛋白、葡萄糖转运蛋白、富含亮氨酸重复序列蛋白和氨单加氧酶。对编码乙烯不敏感蛋白的基因（EU071774）和编码氨单加氧酶的基因（EU071781）的表达谱进行实时PCR分析，结果表明这两个基因的表达在PEG和ABA处理下均上调，表明它们可能参与斑茅的抗旱过程。本研究是关于干旱胁迫激活斑茅中基因的首次报道，并开启了cDNA-SRAP在特定胁迫条件下斑茅差异基因表达分析中的应用。
