Rozanski Waldemar, Krzeslak Anna, Forma Ewa, Brys Magdalena, Blewniewski Mariusz, Wozniak Paweł, Lipinski Marek
2nd Department of Urology, Medical University of Lodz, Lodz, Poland.
Clin Lab. 2012;58(5-6):579-83.
The mRNA expression of genes coding enzymes involved in O-GlcNAcylation were analyzed in urine obtained from 176 bladder cancer (BC) patients and 143 healthy persons.
MGEA5 and OGT expression was measured by a real-time PCR assay.
OGT expression was not detected in urine of healthy persons but it was found in 51.7% of BC samples. Positive expression of MGEA5 was found in urine of both healthy persons (47.1%) and BC patients (52.3%). Poorly differentiated BC (grade III) showed significantly lower MGEA5 expression than grade I tumors. Contrary, OGT transcript level was significantly higher in grade II and III in comparison to grade I BC. Moreover, there was significant difference in OGT expression between early bladder cancers and invasive or advanced bladder cancers.
These results suggest that analysis of urinary content of MGEA5 and OGT may be useful for bladder cancer diagnostics.
对176例膀胱癌（BC）患者和143名健康人的尿液中编码参与O-连接N-乙酰葡糖胺化（O-GlcNAcylation）的酶的基因的mRNA表达进行了分析。
通过实时PCR测定法测量MGEA5和OGT的表达。
在健康人的尿液中未检测到OGT表达，但在51.7%的BC样本中发现了OGT表达。在健康人（47.1%）和BC患者（52.3%）的尿液中均发现了MGEA5的阳性表达。低分化BC（III级）的MGEA5表达明显低于I级肿瘤。相反，与I级BC相比，II级和III级的OGT转录水平明显更高。此外，早期膀胱癌与浸润性或晚期膀胱癌之间的OGT表达存在显著差异。
这些结果表明，分析尿液中MGEA5和OGT的含量可能有助于膀胱癌的诊断。
