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环磷酸腺苷对大肠杆菌中噬菌体T6/大肠杆菌素K受体的调控

Cyclic adenosine 3',5'-monophosphate regulation of the bacteriophage T6/colicin K receptor in Escherichia coli.

作者信息

Alderman E M, Dills S S, Melton T, Dobrogosz W J

出版信息

J Bacteriol. 1979 Nov;140(2):369-76. doi: 10.1128/jb.140.2.369-376.1979.

Abstract

Mutant strains of Escherichia coli unable to synthesize cyclic adenosine 3',5'-monophosphate (cAMP) or the cyclic adenosine monophosphate receptor protein (CRP) were more resistant than wild-type cells to infection by bacteriophage T6. This resistance was found to be associated with the decreased production of specific T6 receptor protein (also the colicin K receptor) located in the outer membrane protein fraction of these cells. Transcription of this particular outer membrane protein was regulated by the cAMP-CRP complex. A novel affinity technique coupled with sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used in these investigations.

摘要

无法合成3',5'-环磷酸腺苷(cAMP)或环磷酸腺苷受体蛋白(CRP)的大肠杆菌突变株比野生型细胞对噬菌体T6的感染更具抗性。发现这种抗性与这些细胞外膜蛋白组分中特定T6受体蛋白(也是大肠杆菌素K受体)产量的降低有关。这种特定外膜蛋白的转录受cAMP-CRP复合物调控。在这些研究中使用了一种结合十二烷基硫酸钠-聚丙烯酰胺凝胶电泳的新型亲和技术。

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