Key Laboratory of Advanced Technology for Materials of Education Ministry, Southwest Jiaotong University, Chengdu 610031, China.
Biomaterials. 2012 Oct;33(28):6615-25. doi: 10.1016/j.biomaterials.2012.06.055. Epub 2012 Jul 9.
In this work, the effects of a heparin-functionalized coating on the growth behavior of vascular cells were studied. To retain its functionality, heparin was bound to a cationic plasma polymerized allylamine coating through electrostatic interaction. The heparin binding surface significantly inhibited human umbilical artery smooth muscle cell (HUASMC) adhesion and proliferation. In contrast, human umbilical vein endothelial cells (HUVECs) showed significant enhancement in cell adhesion, proliferation and migration, release of nitric oxide (NO) and secretion of prostaglandin I(2) (PGI(2)). The test of acute thrombogenicity assessed using human blood exhibited an excellent antithrombotic performance of the heparin grafted surface. The heparinized surface significantly promoted in vivo re-endothelialization and effectively inhibited thrombosis formation. These observations form an important framework for further deciphering the biological functions of heparin. It is highlighted that these striking findings may serve as a guide for the design of multifunctional vascular devices.
本工作研究了肝素官能化涂层对血管细胞生长行为的影响。为了保持其功能，肝素通过静电相互作用结合到阳离子等离子体聚合的丙烯胺涂层上。肝素结合表面显著抑制人脐动脉平滑肌细胞（HUASMC）的黏附和增殖。相比之下，人脐静脉内皮细胞（HUVEC）的黏附、增殖和迁移、一氧化氮（NO）的释放和前列环素 I（2）（PGI（2））的分泌显著增强。使用人血评估的急性血栓形成试验显示肝素接枝表面具有优异的抗血栓性能。肝素化表面显著促进体内再内皮化并有效抑制血栓形成。这些观察结果为进一步解析肝素的生物学功能提供了重要框架。值得强调的是，这些显著的发现可能为多功能血管器械的设计提供指导。
