Key Laboratory of Advanced Technology for Materials of Education Ministry, ‡The Institute of Biomaterials and Surface Engineering, School of Materials Science and Engineering, and §Laboratory of Biosensing and MicroMechatronics, Southwest Jiaotong University , Chengdu 610031, China.
ACS Appl Mater Interfaces. 2014 Feb 26;6(4):2647-56. doi: 10.1021/am405124z. Epub 2014 Feb 6.
The creation of a platform for enhanced vascular endothelia cell (VEC) growth while suppressing vascular smooth muscle cell (VSMC) proliferation offers possibility for advanced coatings of vascular stents. Gallic acid (GA), a chemically unique phenolic acid with important biological functions, presents benefits to the cardiovascular disease therapy because of its superior antioxidant effect and a selectivity to support the growth of ECs more than SMCs. In this study, GA was explored to tailor such a multifunctional stent surface combined with plasma polymerization technique. On the basis of the chemical coupling reaction, GA was bound to an amine-group-rich plasma-polymerized allylamine (PPAam) coating. The GA-functionalized PPAam (GA-PPAam) surface created a favorable microenvironment to obtain high ECs and SMCs selectivity. The GA-PPAam coating showed remarkable enhancement in the adhesion, viability, proliferation, migration, and release of nitric oxide (NO) of human umbilical vein endothelial cells (HUVECs). The GA-PPAam coating also resulted in remarkable inhibition effect on human umbilical artery smooth muscle cell (HUASMC) adhesion and proliferation. These striking findings may provide a guide for designing the new generation of multifunctional vascular devices.
开发一种能够促进血管内皮细胞（VEC）生长同时抑制血管平滑肌细胞（VSMC）增殖的平台，为血管支架的高级涂层提供了可能。没食子酸（GA）是一种具有重要生物学功能的化学独特酚酸，由于其卓越的抗氧化作用和选择性支持 EC 生长而不是 SMC 生长，对心血管疾病治疗具有益处。在这项研究中，GA 被探索用于结合等离子体聚合技术定制这种多功能支架表面。基于化学偶联反应，GA 被结合到富含胺基团的等离子体聚合丙烯胺（PPAam）涂层上。GA 功能化的 PPAam（GA-PPAam）表面创造了有利的微环境，获得了高 ECs 和 SMCs 的选择性。GA-PPAam 涂层显著增强了人脐静脉内皮细胞（HUVECs）的黏附、活力、增殖、迁移和一氧化氮（NO）释放。GA-PPAam 涂层还对人脐动脉平滑肌细胞（HUASMC）的黏附和增殖具有显著的抑制作用。这些显著的发现可能为设计新一代多功能血管设备提供指导。
