Nikolova E, Staykova M, Raicheva D, Karadjova M, Neronov A, Ivanov I, Goranov I
Institute of Cell Biology and Morphology, Bulgarian Academy of Sciences, Sofia.
Am J Reprod Immunol. 1990 Aug;23(4):104-6. doi: 10.1111/j.1600-0897.1990.tb00681.x.
Human colostral cells were pulsed with PHA, Con A, or LPS and cultivated in serum-free medium. The culture supernatants were tested for IL-1 activity in C3H/HeJ thymocyte assay and for IL-2 activity on human lymphoblasts. The IL-1 activity was the highest at the 24th h of cultivation and IL-2 activity at the 48th h of cultivation.
用人初乳细胞用PHA、刀豆蛋白A或脂多糖进行脉冲处理，并在无血清培养基中培养。在C3H/HeJ胸腺细胞试验中检测培养上清液的IL-1活性，在人淋巴母细胞上检测IL-2活性。培养24小时时IL-1活性最高，培养48小时时IL-2活性最高。
