通过电子自旋共振光谱法测量多形核白细胞产生的羟基自由基。
Hydroxyl radical generation by polymorphonuclear leukocytes measured by electron spin resonance spectroscopy.
作者信息
Rosen H, Klebanoff S J
出版信息
J Clin Invest. 1979 Dec;64(6):1725-9. doi: 10.1172/JCI109637.
Abstract
Electron spin resonance spectroscopy using the spin trap 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) was employed to detect the formation of hydroxyl radicals (OH.) by phagocytosing polymorphonuclear leukocytes (PMN). An electron spin resonance signal with the identical g value and splitting characteristics of the DMPO/OH). adduct was detected on incubation of normal PMN with opsonized zymosan. Adduct formation was strongly inhibited by superoxide dismutase and by the OH. scavenger mannitol, but catalase had little or no effect. (DMPO/OH). was not formed by PMN from a patient with chronic granulomatous disease; in contrast, adduct formation by PMN which lack myeloperoxidase was greater than normal. These findings are discussed in relation to the formation of OH. by PMN.
摘要
使用自旋捕获剂5,5-二甲基-1-吡咯啉-N-氧化物（DMPO）的电子自旋共振光谱法用于检测吞噬多形核白细胞（PMN）产生的羟基自由基（OH·）。在用调理酵母聚糖孵育正常PMN时，检测到具有与DMPO/OH·加合物相同g值和分裂特征的电子自旋共振信号。加合物的形成受到超氧化物歧化酶和OH·清除剂甘露醇的强烈抑制，但过氧化氢酶几乎没有影响或没有影响。慢性肉芽肿病患者的PMN不会形成DMPO/OH·；相反，缺乏髓过氧化物酶的PMN形成的加合物比正常情况更多。结合PMN产生OH·的情况对这些发现进行了讨论。
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