Iizuka M, Chiba M, Horie Y, Masamune O, Ohta H
First Department of Internal Medicine, Akita University School of Medicine, Japan.
Gastroenterol Jpn. 1990 Dec;25(6):700-7. doi: 10.1007/BF02779183.
Lymphoid cell subsets, including T cells as well as Ig-containing cells in the colonic mucosa and HLA-DR antigens on colonic epithelia, were examined in non-IBD colitis (colitis excluding ulcerative colitis (UC) and Crohn's disease) by the indirect immunoperoxidase staining method. Mouse anti-CD5, CD8, CD4, IgG, IgA1, IgA2, IgM, IgD, IgE, HLA-DR, and NuIa monoclonal antibodies were used as the first antibody. The results were compared to those of the normal controls and UC. T cell subsets in non-IBD colitis were almost similar to those of the controls and UC. The number of Ig-containing cells of all classes, except for IgA, tended to be increased in non-IBD colitis. In particular, both IgG- and IgE-containing cells were significantly increased compared to those in the controls. Compared to UC, IgG-containing cells were decreased in non-IBD colitis. Namely, in non-IBD colitis, as well as in UC, the change of Ig-containing cells (B cell lineage) was more pronounced than that of T cells. The frequency of the expression of HLA-DR antigens on colonic epithelia in non-IBD colitis was 70%, which was significantly higher than that in controls (0%), but significantly lower than that in UC (100%). Whether the differences in the number of IgG-containing cells, and the frequency of epithelial HLA-DR expression between non-IBD colitis and UC was due to the differences of the degree of local inflammation or due to the differences of the nature of the two diseases was not elucidated in this study.
采用间接免疫过氧化物酶染色法，对非炎症性肠病性结肠炎（不包括溃疡性结肠炎（UC）和克罗恩病的结肠炎）患者结肠黏膜中的淋巴细胞亚群（包括T细胞以及含免疫球蛋白的细胞）和结肠上皮细胞上的HLA - DR抗原进行了检测。使用小鼠抗CD5、CD8、CD4、IgG、IgA1、IgA2、IgM、IgD、IgE、HLA - DR和NuIa单克隆抗体作为一抗。将结果与正常对照和UC患者的结果进行比较。非炎症性肠病性结肠炎中的T细胞亚群与对照和UC患者的T细胞亚群几乎相似。除IgA外，所有类型的含免疫球蛋白细胞数量在非炎症性肠病性结肠炎中均有增加趋势。特别是，与对照组相比，含IgG和IgE的细胞显著增加。与UC相比，非炎症性肠病性结肠炎中含IgG的细胞减少。也就是说，在非炎症性肠病性结肠炎以及UC中，含免疫球蛋白细胞（B细胞系）的变化比T细胞更为明显。非炎症性肠病性结肠炎患者结肠上皮细胞上HLA - DR抗原的表达频率为70%，显著高于对照组（0%），但显著低于UC患者（100%）。本研究未阐明非炎症性肠病性结肠炎与UC之间含IgG细胞数量以及上皮HLA - DR表达频率的差异是由于局部炎症程度不同还是由于两种疾病本质不同所致。
