UMR 7598 LJLL, BC187, Université Pierre et Marie Curie-Paris 6, 4 Place de Jussieu, F-75252 Paris Cedex 5, France.
J Theor Biol. 2012 Oct 21;311:19-27. doi: 10.1016/j.jtbi.2012.07.001. Epub 2012 Jul 11.
Cells grown in culture act as a model system for analyzing the effects of anticancer compounds, which may affect cell behavior in a cell cycle position-dependent manner. Cell synchronization techniques have been generally employed to minimize the variation in cell cycle position. However, synchronization techniques are cumbersome and imprecise and the agents used to synchronize the cells potentially have other unknown effects on the cells. An alternative approach is to determine the age structure in the population and account for the cell cycle positional effects post hoc. Here we provide a formalism to use quantifiable lifespans from live cell microscopy experiments to parameterize an age-structured model of cell population response.
在培养的细胞中，作为分析抗癌化合物影响的模型系统，这些化合物可能以细胞周期位置依赖的方式影响细胞行为。通常采用细胞同步化技术来最小化细胞周期位置的变化。然而，同步化技术繁琐且不精确，并且用于同步化细胞的试剂可能对细胞产生其他未知的影响。另一种方法是确定群体中的年龄结构，并在事后考虑细胞周期位置的影响。在这里，我们提供了一种形式主义，利用活细胞显微镜实验中的可量化寿命来参数化细胞群体反应的年龄结构模型。
