Division of Experimental Orthopaedics, Orthopaedic University Hospital of Heidelberg, Heidelberg, Germany.
Cells Tissues Organs. 2012;196(6):523-33. doi: 10.1159/000337490. Epub 2012 Jul 10.
Both platelet-rich plasma (PRP) and vascular endothelial growth factor (VEGF) can promote regeneration. The aim of this study was to compare the effects of these two elements on bone formation and vascularization in combination with bone marrow stromal cells (BMSC) in a critical-size bone defect in rabbits. The critical-size defects of the radius were filled with: (1) a calcium-deficient hydroxyapatite (CDHA) scaffold + phVEGF(165)-transfected BMSC (VEGF group), (2) CDHA and PRP, or (3) CDHA, autogenous BMSC, and PRP. As controls served: (4) the CDHA scaffold alone and (5) the CDHA scaffold and autogenous BMSC. The volume of new bone was measured by means of micro-CT scans, and vascularization was assessed in histology after 16 weeks. Bone formation was higher in the PRP + CDHA, BMSC + CDHA, and PRP + BMSC + CDHA groups than in the VEGF group (p < 0.05). VEGF transfection significantly promoted vascularization of the scaffolds in contrast to BMSC and PRP (p < 0.05), but was similar to the result of the CDHA + PRP + BMSC group. The results show that VEGF-transfected BMSC as well as the combination of PRP and BMSC improve vascularization, but bone healing was better with the combination of BMSC and PRP than with VEGF-transfected BMSC. Expression of VEGF in BMSC as a single growth factor does not seem to be as effective for bone formation as expanded BMSC alone or PRP which contains a mixture of growth factors.
富血小板血浆 (PRP) 和血管内皮生长因子 (VEGF) 均可促进再生。本研究旨在比较这两种物质与骨髓基质细胞 (BMSC) 联合应用于兔临界尺寸骨缺损时对成骨和血管生成的影响。用以下方法填充桡骨临界尺寸缺损：(1) 缺钙羟基磷灰石 (CDHA) 支架+转染的 phVEGF(165)-BMSC (VEGF 组)，(2) CDHA 和 PRP，或 (3) CDHA、自体 BMSC 和 PRP。作为对照：(4) 单独 CDHA 支架和 (5) 单独 CDHA 支架和自体 BMSC。通过 micro-CT 扫描测量新骨体积，16 周后在组织学上评估血管化。PRP+CDHA、BMSC+CDHA 和 PRP+BMSC+CDHA 组的骨形成量高于 VEGF 组(p<0.05)。与 BMSC 和 PRP 相比，VEGF 转染显著促进了支架的血管生成(p<0.05)，但与 CDHA+PRP+BMSC 组相似。结果表明，VEGF 转染的 BMSC 以及 PRP 和 BMSC 的联合应用可改善血管化，但 BMSC 和 PRP 的联合应用比 VEGF 转染的 BMSC 更有利于骨愈合。作为单一生长因子的 BMSC 中 VEGF 的表达似乎不如单独扩增的 BMSC 或含有多种生长因子的 PRP 对成骨有效。
