Key Lab of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, Jiangsu, People's Republic of China.
Antonie Van Leeuwenhoek. 2013 Jul;104(1):123-8. doi: 10.1007/s10482-013-9932-2. Epub 2013 May 8.
A bacterial strain, designated J6(T), was isolated from activated sludge, collected from a chemical wastewater treatment system in Zhejiang Province of China. The cells stained Gram-negative, were aerobic, pale-yellow, and non-motile short rods. Phylogenetic analysis of the 16S rRNA gene sequence indicated that the closest relative of this organism was Paracoccus aminophilus KACC 12262(T) = JCM 7686(T) (97.4 % sequence similarity). Strain J6(T) grew at 10-37 °C (optimum 30 °C), at pH 6.0-8.0 (optimum pH 7.0) and with 0-5 % NaCl (optimum 3 %, w/v). The predominant cellular fatty acid found was summed feature 8(C18:1 ω7c and/or C18:1 ω6c; 82.8 %). The major respiratory quinone-detected was Q-10 and the DNA G+C content was 61.9 mol %. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and several unknown polar lipids. Strain J6(T) showed low DNA-DNA relatedness values with P. aminophilus KACC 12262(T) (28 ± 3 %). The phylogenetic analysis, DNA-DNA hybridization, whole-cell fatty acid composition as well as biochemical characteristics allowed clear differentiation of the isolate from the other type strains of already described Paracoccus species. It is evident from the genotypic, phenotypic and chemotaxonomic analyses that strain J6(T) should be classified as a novel species of the genus Paracoccus, for which the name P. zhejiangensis sp. nov. is proposed. The type strain is J6(T) (KACC 16703(T) = CCTCC AB 2012031(T)).
一株细菌菌株，命名为 J6(T)，从浙江省某化工厂废水处理系统的活性污泥中分离得到。细胞革兰氏染色阴性，为需氧、浅黄色、非运动短杆菌。16S rRNA 基因序列的系统发育分析表明，该菌与 Paracoccus aminophilus KACC 12262(T) = JCM 7686(T)（相似度 97.4%）最为接近。菌株 J6(T)在 10-37°C（最适 30°C）、pH6.0-8.0（最适 pH7.0）和 0-5%NaCl（最适 3%，w/v）的条件下生长。发现的主要细胞脂肪酸为总和特征 8（C18:1 ω7c 和/或 C18:1 ω6c；82.8%）。检测到的主要呼吸醌是 Q-10，DNA G+C 含量为 61.9mol%。极性脂谱由磷脂酰乙醇胺、磷脂酰甘油、双磷脂酰甘油、磷脂酰胆碱和几种未知极性脂组成。菌株 J6(T)与 P. aminophilus KACC 12262(T)（28±3%）的 DNA-DNA 同源性值较低。系统发育分析、DNA-DNA 杂交、全细胞脂肪酸组成以及生化特性均能明确区分该分离株与已描述的 Paracoccus 属其他种的模式株。综合基因型、表型和化学分类特征分析，菌株 J6(T)应归类为 Paracoccus 属的一个新种，现命名为 Paracoccus zhejiangensis sp. nov.。模式株为 J6(T)（KACC 16703(T) = CCTCC AB 2012031(T)）。
