Biomaterials and Biocatalysts Group, Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore 117604.
Korean Agricultural Culture Collection (KACC), Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration (RDA), Suwon 441-707, Republic of Korea.
Int J Syst Evol Microbiol. 2013 Apr;63(Pt 4):1241-1248. doi: 10.1099/ijs.0.039685-0. Epub 2012 Jul 13.
A short rod-shaped Gram-stain-positive actinobacterium was isolated as an endophyte from the tissues of Jatropha curcas cv. KB27 and was investigated by means of a polyphasic taxonomic approach. An analysis of its 16S rRNA gene sequence indicated that strain S9-650(T) forms an individual line of descent and is related to certain members of the suborder Frankineae, order Actinomycetales (<95 % sequence similarity). Distance-matrix and neighbour-joining analyses set the branching point of the novel isolate between two clades, one being represented by members of the genera Frankia (family Frankiaceae) and Acidothermus (family Acidothermaceae) and the other by members of the genera Geodermatophilus, Blastococcus and Modestobacter (family Geodermatophilaceae). The organism had meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. The acyl type was found to be N-glycolylated. The major menaquinone was MK-9(H4) and the fatty acid profile was characterized by the predominance of iso-C16 : 0, C18 : 1ω9c, anteiso-C17 : 0 and C17 : 1ω8c. The polar lipids comprised diphosphatidylglycerol, an unidentified glycolipid, phospholipids and aminolipids. The G+C content of the genomic DNA was 71.2 mol%. The distinct phylogenetic position and the phenotypic markers that clearly separate the novel organism from all other members of the suborder Frankineae indicate that strain S9-650(T) represents a novel species in a new genus, for which the name Jatrophihabitans endophyticus gen. nov., sp. nov. is proposed. The type strain of the type species is S9-650(T) ( = DSM 45627(T) = KACC 16232(T)).
从 Jatropha curcas cv. KB27 的组织中分离出一株短杆状革兰氏阳性放线菌，作为内生菌进行了多相分类学研究。16S rRNA 基因序列分析表明，菌株 S9-650(T)形成一个独立的进化枝，与某些弗兰克亚目的成员有关，属于放线菌目 (<95%序列相似性)。距离矩阵和邻接法分析将新分离株的分支点置于两个分支之间，一个分支由弗兰克氏菌属（弗兰克氏菌科）和嗜酸热菌属（嗜酸热菌科）的成员代表，另一个分支由地衣芽孢杆菌属、芽胞杆菌属和莫德斯托杆菌属（地衣芽孢杆菌科）的成员代表。该菌细胞壁肽聚糖中的特征二氨基庚二酸为meso-二氨基庚二酸。酰基类型被发现是 N-糖基化的。主要的menaquinone 是 MK-9(H4)，脂肪酸图谱的特点是 iso-C16:0、C18:1ω9c、anteiso-C17:0 和 C17:1ω8c 占优势。极性脂包括双磷脂酰甘油、一种未鉴定的糖脂、磷脂和氨基脂。基因组 DNA 的 G+C 含量为 71.2 mol%。新菌株独特的系统发育位置和与弗兰克亚目所有其他成员明显分离的表型标记表明，S9-650(T) 代表了一个新属的新种，建议将其命名为 Jatrophihabitans endophyticus 属，种。模式种的模式菌株是 S9-650(T)（=DSM 45627(T)=KACC 16232(T)）。
