Zhang S, Shi Z, Zhang J, Bonami J-R
Joint-Laboratory of Invertebrate Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
J Fish Dis. 2004 Dec;27(12):687-92. doi: 10.1111/j.1365-2761.2004.00587.x.
A new reovirus was recently isolated from a freshwater crab, the Chinese mitten crab, Eriocheir sinensis, in China. The complete viral particles are 55 nm in diameter, icosahedral, non-enveloped and have a mean buoyant density of 1.39 g cm(-3) in CsCl gradient. The viral genome is composed of 12 pieces of dsRNA with an electrophoretic pattern of 3/4/2/3. This virus infects connective tissue of the gills, gut and hepatopancreas. Partial cDNA cloning and sequence analysis showed that the RNA-dependent RNA polymerase is located in the first RNA segment. From its biochemical, ultrastructural and physicochemical properties, this virus is quite different from the genus Aquareovirus (Reoviridae). It may represent a new genus of Reoviridae, different from the other crab reoviruses, P and W(2).
最近在中国从淡水蟹中华绒螯蟹中分离出一种新型呼肠孤病毒。完整病毒粒子直径55纳米，呈二十面体，无包膜，在CsCl梯度中的平均浮力密度为1.39克/立方厘米。病毒基因组由12条双链RNA组成，电泳图谱为3/4/2/3。该病毒感染鳃、肠道和肝胰腺的结缔组织。部分cDNA克隆和序列分析表明，依赖RNA的RNA聚合酶位于第一个RNA片段中。从其生化、超微结构和物理化学性质来看，这种病毒与水生呼肠孤病毒属（呼肠孤病毒科）有很大不同。它可能代表呼肠孤病毒科的一个新属，不同于其他蟹呼肠孤病毒P和W(2)。
