从食源性蜡样芽胞杆菌分离株中检测和测序质粒编码的四环素耐药决定因子（tetA 和 tetB）。
Detection and sequencing of plasmid encoded tetracycline resistance determinants (tetA and tetB) from food-borne Bacillus cereus isolates.
机构信息
Division of Veterinary Public Health, Faculty of Veterinary Sciences and Animal Husbandry, SKUAST-K, J&K, India.
出版信息
Asian Pac J Trop Med. 2012 Sep;5(9):709-12. doi: 10.1016/S1995-7645(12)60111-4.
OBJECTIVE
To investigate the detection and sequencing of plasmid encoded tetracycline resistance genes (tetA and tetB) from food-borne and standard strains of Bacillus cereus (B. cereus).
METHODS
A PCR was carried out to detect the tetracycline resistance genes (tetA and tetB) in food-borne B. cereus strains and the amplified products were sequenced.
RESULTS
The phenotypic resistance against tetracycline was observed in 39 of the 118 food-borne isolates and two reference strains (MTCC 430 and MTCC 1307) of B. cereus. Among the phenotypically resistant isolates, tetA was detected in 36 food-borne isolates and two reference strains (MTCC 430 and MTCC 1307), whereas, tetB was detected in 12 food-borne isolates and MTCC 1307 strain.
CONCLUSIONS
A close association was therefore found between phenotypic resistance against tetracycline and presence of tetracycline resistance genes. The tetA and tetB gene fragments were amplified, purified and sequenced. The gene sequences of the isolates studied herein were found similar to tetA and tetB gene sequences of other bacteria available in NCBI. The occurrence of tetA and tetB genes in B. cereus indicate the horizontal transfer of antibiotic resistance determinants from other bacteria into B. cereus. The transfer of these resistant determinants to other potentially pathogenic bacteria may be a matter of great concern.
目的
从食源性和标准蜡样芽胞杆菌（B. cereus）菌株中检测和测序质粒编码的四环素耐药基因（tetA 和 tetB）。
方法
采用 PCR 方法检测食源性 B. cereus 菌株中的四环素耐药基因（tetA 和 tetB），并对扩增产物进行测序。
结果
在 118 株食源性分离株和 2 株蜡样芽胞杆菌参考株（MTCC 430 和 MTCC 1307）中，有 39 株表现出对四环素的表型耐药性。在表型耐药的分离株中，36 株食源性分离株和 2 株参考株（MTCC 430 和 MTCC 1307）中检测到 tetA，而 12 株食源性分离株和 MTCC 1307 株中检测到 tetB。
结论
因此，四环素表型耐药与四环素耐药基因的存在之间存在密切关联。扩增、纯化和测序了 tetA 和 tetB 基因片段。研究中分离株的基因序列与 NCBI 中其他细菌的 tetA 和 tetB 基因序列相似。B. cereus 中 tetA 和 tetB 基因的存在表明抗生素耐药决定因素从其他细菌水平转移到 B. cereus 中。这些耐药决定因素转移到其他潜在的致病性细菌中可能是一个非常值得关注的问题。
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