The SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines and the MOE Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, 201210, China.
World J Microbiol Biotechnol. 2012 Apr;28(4):1803-6. doi: 10.1007/s11274-011-0934-9. Epub 2011 Nov 6.
Biotransformation of naringenin with Aspergillus niger CGMCC 3.4628 yielded two hydroxylation products which were identified unambiguously as 6-hydroxylnaringenin (carthamidin) and 8-hydroxylnaringenin (isocarthamidin) by ESI-MS and (1)H-NMR. Both products simultaneously arrived at high level after 48 h in the biotransformation process. The highest conversion efficiency of carthamidin was 0.38 mg/mg of naringenin and that of isocarthamidin was 0.43 mg/mg of naringenin. Antioxidant property assay using a thin layer chromatography-bioautographic-based DPPH scavenging method demonstrated that both hydroxylation metabolites exhibited much stronger activity than naringenin. The high efficiency and convenient procedure makes the biotransformation with A. niger described in current work a potential way to produce carthamidin and isocarthamidin.
黑曲霉 CGMCC 3.4628 对柚皮苷的生物转化生成了两种羟化产物，通过 ESI-MS 和（1）H-NMR 可以明确鉴定这两种产物分别为 6-羟基柚皮苷（芦丁）和 8-羟基柚皮苷（异芦丁）。在生物转化过程中，这两种产物在 48 小时后同时达到高水平。芦丁的最高转化率为 0.38mg/mg 柚皮苷，异芦丁的转化率为 0.43mg/mg 柚皮苷。采用薄层色谱-生物自显影法测定 DPPH 清除法的抗氧化活性表明，两种羟化代谢产物的活性均明显强于柚皮苷。该方法高效、便捷，用黑曲霉进行生物转化可能是生产芦丁和异芦丁的一种有前途的方法。
