The State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, China.
Am J Chin Med. 2012;40(4):861-75. doi: 10.1142/S0192415X12500644.
Pinus massoniana bark extract (PMBE) is a mixture of flavonoids, and showed a capability of inducing cell apoptosis; however, its properties have not yet been fully investigated. This paper evaluates the antitumor effects of PMBE in murine sarcoma S180 both in vitro and in vivo. In vitro, the growth inhibition of S180 cells was concentration dependent on PMBE as shown by the CCK-8 assay. The AO/EB staining and flow cytometry assay showed that PMBE induced S180 cell apoptosis. Cell cycle analysis revealed that the cells in the S phase were decreased by treatment with PMBE. In vivo, the treatment of 100, 200, and 300 mg/kg PMBE reduced the tumor weight and volume of S180-bearing NIH mice by 9%-67% and 13%-68%, respectively. Peripheral leukocyte count and lymphoproliferation were increased significantly after treatment with PMBE. Our results suggest that PMBE inhibits the tumor cell growth by inducing cell apoptosis and improving lymphoproliferation.
马尾松皮提取物（PMBE）是一种黄酮类混合物，具有诱导细胞凋亡的能力；然而，其性质尚未得到充分研究。本文评估了 PMBE 在体外和体内对小鼠肉瘤 S180 的抗肿瘤作用。在体外，CCK-8 检测结果表明，PMBE 对 S180 细胞的生长抑制作用呈浓度依赖性。AO/EB 染色和流式细胞术检测结果表明，PMBE 诱导 S180 细胞凋亡。细胞周期分析显示，PMBE 处理后 S 期细胞减少。在体内，100、200 和 300mg/kg PMBE 处理可分别使荷瘤 NIH 小鼠的肿瘤重量和体积减少 9%-67%和 13%-68%。PMBE 处理后外周白细胞计数和淋巴细胞增殖明显增加。我们的结果表明，PMBE 通过诱导细胞凋亡和改善淋巴细胞增殖来抑制肿瘤细胞生长。
