Mao Ping, Zhang Ershao, Chen Yang, Liu Likun, Rong Daqing, Liu Qingfeng, Li Weiling
Department of General Surgery, The People's Hospital of Liaoning Province, Shenyang, Liaoning 110016, P.R. China.
Department of Biotechnology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
Oncol Lett. 2017 Feb;13(2):1019-1023. doi: 10.3892/ol.2016.5509. Epub 2016 Dec 16.
The bark of Pinus massoniana is a traditional Chinese medicine for the treatment of various health disorders. Previous studies have demonstrated that P. massoniana bark extract (PMBE) may induce the apoptosis of hepatoma and cervical cancer cells. However, whether PMBE is able to inhibit the migration of lung cancer cells requires further investigation. In the current study, the effects of PMBE on the viability of human lung cancer A549 cells were detected using an MTT assay. The migration of lung cancer cells following exposure to PMBE were quantified using wound healing and Transwell assays, respectively. The expression levels of matrix metalloproteinase (MMP)-9 were determined using western blotting. The results revealed that PMBE significantly inhibited the growth of the lung cancer cells. In addition, the wound closure rate and the migration of the lung cancer cells were suppressed by PMBE. Furthermore, the expression levels of MMP-9 were reduced. These findings indicated that PMBE is able to restrict the migration and invasion of lung cancer cells, and that PMBE may serve as a novel therapeutic agent for patients with metastatic lung cancer in the future.
马尾松的树皮是一种用于治疗各种健康疾病的传统中药。先前的研究表明，马尾松树皮提取物（PMBE）可能诱导肝癌和宫颈癌细胞凋亡。然而，PMBE是否能够抑制肺癌细胞的迁移需要进一步研究。在当前研究中，使用MTT法检测PMBE对人肺癌A549细胞活力的影响。分别使用伤口愈合试验和Transwell试验对暴露于PMBE后的肺癌细胞迁移进行定量。使用蛋白质印迹法测定基质金属蛋白酶（MMP）-9的表达水平。结果显示，PMBE显著抑制肺癌细胞的生长。此外，PMBE抑制了伤口闭合率和肺癌细胞的迁移。此外，MMP-9的表达水平降低。这些发现表明，PMBE能够限制肺癌细胞的迁移和侵袭，并且PMBE未来可能成为转移性肺癌患者的一种新型治疗药物。
