[特定核酸内切酶R. HpaI、R. EcoRI、R. HindIII对鸡腺病毒CELO的DNA片段化作用]
[DNA fragmentation of chicken adenovirus CELO by specific endonucleases R. HpaI, R. EcoRI, R. HindIII].
作者信息
Denisova T S, Sitnikov B S, Gibadulin R A
出版信息
Mol Biol (Mosk). 1979 Sep-Oct;13(5):1021-34.
PMID:228178
Abstract
The effect of specific endonucleases on DNA chiken adenovirus CELO was studed. It was shown that endonucleases R. HpaI, R. EcoRI and R. HindIII cleaved viral DNA into 5,7 and 9 specific fragments, respectively. The sequence of fragments (physical map) was determined and found to be: D-A-E-C-B for enzyme R. HpaI; B-(EG)-C-A-D-F for enzyme R. EcoRI; H-F-A-C-G-B-D-E-I for enzyme R. HindIII.
摘要
研究了特定核酸内切酶对鸡腺病毒CELO DNA的作用。结果表明，核酸内切酶R. HpaI、R. EcoRI和R. HindIII分别将病毒DNA切割成5个、7个和9个特定片段。确定了片段序列（物理图谱），发现对于R. HpaI酶为：D-A-E-C-B；对于R. EcoRI酶为：B-(EG)-C-A-D-F；对于R. HindIII酶为：H-F-A-C-G-B-D-E-I。
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