Kondo Z, Makino T, Iizuka R
Department of Obstetrics and Gynecology, School of Medicine, Keio University, Tokyo, Japan.
J Clin Lab Anal. 1990;4(6):410-3. doi: 10.1002/jcla.1860040604.
Measurement of Estriol (E3) and estradiol (E2) within 22 min by high-performance liquid chromatography (HPLC) was achieved in this study, and the values were compared with those of a radioimmunoassay (RIA). A totally computerized HPLC method was developed for measuring unconjugated E3 (u-E3) and E2 (u-E2) in the sera of pregnant women. The serum samples were injected directly into the apparatus and transferred to a pretreatment column where the estrogens were absorbed while hydrophilic components such as proteins and carbohydrates were excluded. The estrogens were then passed through another separation column containing a new type of polymer gel. The mobile phase consisted of an acetonitrile-water mixture, and separation was achieved by means of a reversed-phase procedure. The eluate was monitored for fluorescence. All procedures were monitored and controlled with a built-in microcomputer. Serum samples from 97 normal pregnant women at 20-41 wk gestation were simultaneously assayed by HPLC and RIA. The correlations obtained by HPLC and RIA were as follows: u-E3, y = 0.905x - 0.385, with a coefficient of correlation of r = 0.912; for u-E2, y = 0.964x + 5.024, with a coefficient of correlation of r = 0.841 (y, RIA value; x, HPLC value). The quick measurement of u-E3 and u-E2 by HPLC can be a useful method for evaluating fetoplacental function.
本研究实现了通过高效液相色谱法（HPLC）在22分钟内测定雌三醇（E3）和雌二醇（E2），并将这些值与放射免疫分析法（RIA）所得值进行比较。开发了一种完全计算机化的HPLC方法，用于测定孕妇血清中未结合的E3（u-E3）和E2（u-E2）。血清样本直接注入仪器，转移至预处理柱，在该柱中雌激素被吸附，而蛋白质和碳水化合物等亲水性成分被排除。然后，雌激素通过另一个装有新型聚合物凝胶的分离柱。流动相由乙腈-水混合物组成，通过反相程序实现分离。对洗脱液进行荧光监测。所有程序均由内置微型计算机进行监测和控制。对97名妊娠20 - 41周的正常孕妇的血清样本同时采用HPLC和RIA进行检测。HPLC和RIA所得相关性如下：对于u-E3，y = 0.905x - 0.385，相关系数r = 0.912；对于u-E2，y = 0.964x + 5.024，相关系数r = 0.841（y为RIA值；x为HPLC值）。通过HPLC快速测定u-E3和u-E2可为评估胎儿胎盘功能提供一种有用的方法。
