Uchida N, Makino T, Kondo Z, Iizuka R
Nihon Sanka Fujinka Gakkai Zasshi. 1984 Aug;36(8):1249-53.
Measurement of Estriol (E3) and Estradiol (E2) within 25 minutes by high performance liquid chromatography (HPLC) was achieved in this study and the values were compared with those of radioimmunoassay (RIA). A totally computerized HPLC method was developed for measuring unconjugated E3 and E2 in the sera of pregnant women. The sera samples were injected directly into the apparatus and transferred to a pretreatment column where estrogens were absorbed while hydrophilic components such as proteins and carbohydrates were excluded. The estrogens then passed through another separation column containing a new type of polymer gel. The mobile phase consisted of an acetonitrile/water mixture, and separation was achieved by means of a reversed phase mechanism. The eluent was monitored for fluorescence. All these procedures were monitored and controlled with a built-in microcomputer. The sera samples from 61 normal pregnant women at 20 to 41 weeks of pregnancy were simultaneously assayed by HPLC and RIA. The correlation of values obtained from HPLC and RIA was; E3: Y = 0.875X-0.172, the coefficient of correlation was 0.899, E2: Y = 0.972X + 6.791, the coefficient of correlation was 0.841 (Y = RIA values, X = HPLC values). The quick measurements of E3 and E2 by HPLC can be a useful method in evaluating the feto-placental function.
本研究实现了通过高效液相色谱法（HPLC）在25分钟内测定雌三醇（E3）和雌二醇（E2），并将测定值与放射免疫分析法（RIA）的结果进行比较。开发了一种完全计算机化的HPLC方法，用于测定孕妇血清中未结合的E3和E2。血清样本直接注入仪器，转移至预处理柱，在该柱中雌激素被吸附，而蛋白质和碳水化合物等亲水性成分被排除。然后，雌激素通过另一个装有新型聚合物凝胶的分离柱。流动相由乙腈/水混合物组成，通过反相机制实现分离。对洗脱液进行荧光监测。所有这些操作均由内置微型计算机进行监测和控制。同时采用HPLC和RIA对61例怀孕20至41周的正常孕妇的血清样本进行检测。HPLC和RIA所得值的相关性为：E3：Y = 0.875X - 0.172，相关系数为0.899；E2：Y = 0.972X + 6.791，相关系数为0.841（Y = RIA值，X = HPLC值）。通过HPLC快速测定E3和E2可能是评估胎儿 - 胎盘功能的一种有用方法。
