College of Veterinary Medicine, Konkuk University, #1 Hwayang-dong, Kwangjin-gu, Seoul 143-701, South Korea.
Vet Microbiol. 2012 Dec 28;161(1-2):218-21. doi: 10.1016/j.vetmic.2012.07.010. Epub 2012 Jul 17.
Classical swine fever (CSF) is a contagious viral disease that affects pigs. Wild boars can play an important epidemiological role in CSF outbreaks. In the past decades, studies conducted in many countries have reported that the CSF virus (CSFV) may persist in wild boar populations. The existence of CSFV in the free-ranging wild boar populations was indirectly confirmed by determining the prevalence of antibodies against CSFV in the serum of hunted wild boars. However, analyzing sero-prevalence in hunted wild boars to study the risk of CSF outbreaks is difficult due to insufficient number of samples, limitation of hunting area and biased age distribution of hunted wild boars. To improve this survey method, we collected feces of wild boars from their habitat and tested them using CSFV antibody enzyme-linked immunosorbent assay (ELISA) and CSF virus neutralization (VN) test. In this study, ELISA was found to be highly sensitive for detecting antibodies against CSFV in fecal samples. Most of doubtful or positive results obtained in CSFV ELISA were confirmed by VN tests. Despite the high coincidence rate of antibody-positive samples between CSFV ELISA and VN test, the possibility of false positive reaction should be considered. In the regional distribution, a fact that antibody-positive fecal and serum samples were found in geographically close area was shown. Hence, presence of antibodies in fecal samples may provide vital information regarding the risk of CSF outbreaks in wild boar groups in geographical proximity.
经典猪瘟（Classical swine fever，CSF）是一种传染性病毒病，会影响猪。野猪在 CSF 爆发的流行病学中可能发挥重要作用。在过去几十年中，许多国家的研究报告称，CSFV 可能在野猪种群中持续存在。通过检测被猎杀野猪血清中针对 CSFV 的抗体流行率，间接证实了 CSFV 在自由放养野猪种群中的存在。然而，由于样本数量不足、狩猎区域的限制以及被猎杀野猪的年龄分布存在偏差，分析被猎杀野猪的血清流行率以研究 CSF 爆发的风险具有一定难度。为了改进这种调查方法，我们从野猪栖息地采集粪便，并使用 CSFV 抗体酶联免疫吸附测定（ELISA）和 CSF 病毒中和（VN）试验对其进行检测。在这项研究中，ELISA 被发现对粪便样本中针对 CSFV 的抗体具有很高的敏感性。CSFV ELISA 获得的大多数可疑或阳性结果通过 VN 试验得到了确认。尽管 CSFV ELISA 和 VN 试验的抗体阳性样本之间具有很高的符合率，但仍应考虑假阳性反应的可能性。在区域分布方面，表明在地理位置相近的区域发现了粪便和血清抗体阳性样本。因此，粪便中存在抗体可能为地理上相近的野猪群中 CSF 爆发的风险提供重要信息。
