Department of Microbiology, University Putra Malaysia, Selangor, Malaysia.
Folia Microbiol (Praha). 2013 Mar;58(2):91-101. doi: 10.1007/s12223-012-0183-9. Epub 2012 Jul 29.
The purpose of this study was to improve the survival of Bifidobacterium animalis subsp. lactis 10140 during freeze-drying process by microencapsulation, using a special pediatric prebiotics mixture (galactooligosaccharides and fructooligosaccharides). Probiotic microorganisms were encapsulated with a coat combination of prebiotics-calcium-alginate prior to freeze-drying. Both encapsulated and free cells were then freeze-dried in their optimized combinations of skim milk and prebiotics. Response surface methodology (RSM) was used to produce a coating combination as well as drying medium with the highest cell viability during freeze-drying. The optimum encapsulation composition was found to be 2.1 % Na-alginate, 2.9 % prebiotic, and 21.7 % glycerol. Maximum survival predicted by the model was 81.2 %. No significant (p > 0.05) difference between the predicted and experimental values verified the adequacy of final reduced models. The protection ability of encapsulation was then examined over 120 days of storage at 4 and 25 °C and exposure to a sequential model of infantile GIT conditions including both gastric conditions (pH 3.0 and 4.0, 90 min, 37 °C) and intestinal conditions (pH 7.5, 5 h, 37 °C). Significantly improved cell viability showed that microencapsulation of B. lactis 10140 with the prebiotics was successful in producing a stable symbiotic powdery nutraceutical.
本研究旨在通过微胶囊化提高双歧杆菌亚种。 乳杆菌 10140 在冷冻干燥过程中的存活率，使用特殊的儿科益生元混合物（半乳糖寡糖和低聚果糖）。 益生菌微生物先用益生元-钙-海藻酸钠包衣混合物进行包衣，然后再进行冷冻干燥。 然后将包封细胞和游离细胞在优化的脱脂乳和益生元组合中进行冷冻干燥。 响应面法（RSM）用于生产包衣组合以及在冷冻干燥过程中具有最高细胞活力的干燥介质。 发现最佳包封组成是 2.1%的 Na-海藻酸钠、2.9%的益生元、21.7%的甘油。 预测的最大存活率为 81.2%。 预测值和实验值之间无显著差异（p>0.05）验证了最终简化模型的充分性。 然后在 4°C 和 25°C 下储存 120 天并暴露于婴幼儿胃肠道条件的连续模型（包括胃条件（pH 3.0 和 4.0，90 分钟，37°C）和肠条件（pH 7.5，5 小时，37°C）下，检查包封的保护能力。 显著提高的细胞活力表明，用益生元对 B. lactis 10140 进行微胶囊化成功地生产了稳定的共生粉状营养保健品。
