Charnchai Pattra, Jantama Sirima Suvarnakuta, Prasitpuriprecha Chutinun, Kanchanatawee Sunthorn, Jantama Kaemwich
Metabolic Engineering Research Unit, Institute of Agricultural Technology, School of Biotechnology, Suranaree University of Technology, 111 University Avenue, Suranaree Sub-district, Muang District, Nakhon Ratchasima 30000, Thailand.
Division of Biopharmacy, Faculty of Pharmaceutical Sciences, Ubon Ratchathani University, Warinchamrap, Ubon Ratchathani 34190, Thailand.
PLoS One. 2016 Jun 22;11(6):e0157958. doi: 10.1371/journal.pone.0157958. eCollection 2016.
The viability and functionality of probiotics may be influenced by industrial production processes resulting in a decrease in probiotic efficiency that benefit the health of humans. This study aimed to investigate the probiotic characteristics of Bifidobacterium strains isolated from fecal samples of healthy Thai infants. In the present work, three local strains (BF014, BF052, and BH053) belonging to Bifidobacterium animalis showed a great resistance against conditions simulating the gastrointestinal tract. Among these, B. animalis BF052 possessed considerable probiotic properties, including high acid and bile tolerance, strong adhesion capability to Caco-2 cells, and inhibitory activity against pathogens including Salmonella typhimurium and Vibrio cholerae. This strain also exhibited a high survival rate compared to commercial strains during storage in a wide variety of products, including pasteurized milk, soy milk, drinking yogurt, and orange juice. The impact of food processing processes as well as the freeze-drying process, storage of freeze-dried powders, and incorporation of freeze-dried cells in food matrix on probiotic properties was also determined. The stability of the probiotic properties of the BF052 strain was not affected by food processing chain, especially its resistance in the simulated gastrointestinal conditions and its adherence ability to Caco-2 cells. It indicates that it satisfies the criteria as a potential probiotic and may be used as an effective probiotic starter in food applications.
益生菌的生存能力和功能可能会受到工业生产过程的影响，导致益生菌功效降低，而这些功效对人类健康有益。本研究旨在调查从健康泰国婴儿粪便样本中分离出的双歧杆菌菌株的益生菌特性。在本研究中，三株属于动物双歧杆菌的本地菌株（BF014、BF052和BH053）对模拟胃肠道环境表现出很强的抗性。其中，动物双歧杆菌BF052具有相当多的益生菌特性，包括高耐酸性和耐胆汁性、对Caco-2细胞的强粘附能力以及对包括鼠伤寒沙门氏菌和霍乱弧菌在内的病原体的抑制活性。与商业菌株相比，该菌株在包括巴氏杀菌牛奶、豆浆、饮用酸奶和橙汁在内的多种产品储存期间也表现出较高的存活率。还确定了食品加工过程以及冷冻干燥过程、冷冻干燥粉末的储存以及将冷冻干燥细胞纳入食品基质对益生菌特性的影响。BF052菌株益生菌特性的稳定性不受食品加工链的影响，尤其是其在模拟胃肠道条件下的抗性及其对Caco-2细胞的粘附能力。这表明它符合作为潜在益生菌的标准，可作为食品应用中的有效益生菌发酵剂。
