National Agriculture and Food Research Organization, National Institute of Livestock and Grassland Science, Nasushiobara 329-2793, Japan.
Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Tsukuba, Ibaraki 305-0074, Japan.
Int J Syst Evol Microbiol. 2013 Apr;63(Pt 4):1417-1420. doi: 10.1099/ijs.0.043612-0. Epub 2012 Jul 27.
A taxonomic study was conducted on two Gram-reaction-positive, catalase-negative, irregular short-rod-shaped or coccoid lactic acid bacteria, designated strains SG25(T) and SG23, that were isolated from grains of fermented Japanese rice (Oryza sativa L. subsp. japonica). A phylogenetic analysis based on 16S rRNA gene sequence data clearly showed that the strains belonged to the genus Weissella and were most closely related to Weissella soli LMG 20113(T) (with a sequence similarity of 96.9 % for each novel strain). The peptidoglycan of each strain contained the amino acids glutamic acid, lysine, serine and alanine in a molar ratio of 1.0 : 1.2 : 0.5 : 3.0, respectively. On the basis of the unusual phenotypic characteristics of the novel strains and the low levels of DNA-DNA relatedness recorded between each novel strain and Weissella soli JCM 12536(T), strains SG25(T) and SG23 represent a single novel species in the genus Weissella, for which the name Weissella oryzae sp. nov. is proposed. The type strain is SG25(T) ( = JCM 18191(T) = DSM 25784(T)).
对两株革兰氏阳性、过氧化氢酶阴性、不规则短杆状或球形的乳酸细菌进行了分类学研究，这两株菌分别命名为 SG25(T)和 SG23，它们是从发酵的日本大米（Oryza sativa L. subsp. japonica）的谷物中分离出来的。基于 16S rRNA 基因序列数据分析的系统发育分析清楚地表明，这些菌株属于魏斯氏菌属，与 Weissella soli LMG 20113(T)最为密切相关（每个新菌株的序列相似性为 96.9%）。每个菌株的肽聚糖含有谷氨酸、赖氨酸、丝氨酸和丙氨酸，摩尔比分别为 1.0：1.2：0.5：3.0。基于新型菌株的不寻常表型特征以及每个新型菌株与 Weissella soli JCM 12536(T)之间记录的 DNA-DNA 同源性水平较低，菌株 SG25(T)和 SG23 代表 Weissella 属中的一个单一新型种，建议将其命名为 Weissella oryzae sp. nov.。模式菌株为 SG25(T)（=JCM 18191(T)=DSM 25784(T)）。
