Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Korea.
Int J Syst Evol Microbiol. 2013 Aug;63(Pt 8):2951-2956. doi: 10.1099/ijs.0.047548-0. Epub 2013 Feb 8.
A novel, Gram-stain-positive, non-motile, facultatively anaerobic, rod- or coccoid-shaped bacterium, designated strain ORY33(T), was isolated from the gut of a camel cricket (Diestrammena coreana). The 16S rRNA gene sequence analysis showed that strain ORY33(T) belonged to the genus Weissella, with highest sequence similarity to Weissella koreensis S-5623(T) (97.7 %). The strain grew optimally at 30 °C and pH 7 in the presence of 0 % (w/v) NaCl. Catalase and oxidase activities were negative. The genomic DNA G+C content of strain ORY33(T) was 45.1 mol%. DNA-DNA hybridization values between strain ORY33(T) and closely related members of the genus Weissella were less than 27 %. The major fatty acids of strain ORY33(T) were C18 : 1ω9c, C16 : 0 and C14 : 0. Based on these phenotypic, phylogenetic and genotypic analyses, strain ORY33(T) represents a novel species belonging to the genus Weissella, for which the name Weissella diestrammenae sp. nov. is proposed. The type strain is ORY33(T) (= KACC 16890(T) = JCM 18559(T)).
一株新型的革兰氏阳性、非运动性、兼性厌氧、杆状或球状的细菌，被命名为ORY33(T) 菌株，从一只骆驼蟋蟀（Diestrammena coreana）的肠道中分离得到。16S rRNA 基因序列分析表明，ORY33(T) 菌株属于魏斯氏菌属，与 Weissella koreensis S-5623(T)（97.7%）的序列相似度最高。该菌株在 0%（w/v）NaCl 存在下，于 30°C 和 pH7 条件下生长最佳。该菌株过氧化氢酶和氧化酶活性均为阴性。ORY33(T) 菌株的基因组 DNA G+C 含量为 45.1mol%。ORY33(T) 菌株与魏斯氏菌属中亲缘关系较近的成员之间的 DNA-DNA 杂交值小于 27%。该菌株的主要脂肪酸为 C18:1ω9c、C16:0 和 C14:0。基于这些表型、系统发育和基因型分析，ORY33(T) 菌株代表了魏斯氏菌属中的一个新型物种，被命名为 Weissella diestrammenae sp. nov.。其模式菌株为 ORY33(T)（=KACC 16890(T)=JCM 18559(T)）。
