Wang Huili, Liu Chengyi, Han Jun, Zhen Lin, Zhang Tao, He Xiaoli, Xu Enci, Li Mengqiang
Department of Immunology, Fujian Medical University, Fuzhou, P.R. China.
Oncol Lett. 2012 Aug;4(2):194-198. doi: 10.3892/ol.2012.727. Epub 2012 May 23.
The aim of this study was to evaluate the status of HER2 protein expression in patients with renal cell carcinoma (RCC) and to determine its prognostic significance. A total of 42 paraffin-embedded tumor tissues and 42 additional corresponding adjacent normal tissues from RCC patients were randomly collected and studied using immunohistochemistry (IHC). Protein samples of 6 fresh specimens from tumor and adjacent normal tissues obtained during surgery were extracted and tested using western blotting to confirm the IHC results. Of the 42 tumor tissues and adjacent normal tissues tested, IHC showed that 7 tumors (16.67%) and 33 adjacent normal tissues (78.57%) expressed the HER2 protein. In addition, results of the western blotting revealed weak HER2 reactivity in primary tumor cells in two of 6 specimens obtained during surgery. All 6 normal tissues showed positive expression, which was in accordance with the outcome of IHC. In conclusion, HER2 is frequently expressed in normal renal tissues and rarely expressed in RCC tissues. Furthermore, the HER2 status of normal tissue is negatively correlated with that of the RCC tissues (r=-0.410, P=0.007) and the TNM stage (r=-0.246, P=0.027), suggesting that HER2 is involved in RCC oncogenesis.
本研究的目的是评估肾细胞癌（RCC）患者中HER2蛋白表达的状况，并确定其预后意义。随机收集了42例RCC患者的石蜡包埋肿瘤组织以及另外42例相应的癌旁正常组织，采用免疫组织化学（IHC）进行研究。提取手术中获取的6例肿瘤及癌旁正常组织新鲜标本的蛋白质样本，并用蛋白质印迹法进行检测，以证实IHC结果。在检测的42例肿瘤组织和癌旁正常组织中，IHC显示7例肿瘤（16.67%）和33例癌旁正常组织（78.57%）表达HER2蛋白。此外，蛋白质印迹法结果显示，手术获取的6例标本中有2例原发性肿瘤细胞中的HER2反应较弱。所有6例正常组织均呈阳性表达，这与IHC结果一致。总之，HER2在正常肾组织中频繁表达，而在RCC组织中很少表达。此外，正常组织的HER2状态与RCC组织的HER2状态呈负相关（r=-0.410，P=0.007），与TNM分期呈负相关（r=-0.246，P=0.
