Podkowik M, Bystroń J, Bania J
Department of Food Hygiene and Consumer Health Protection, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Norwida 31, 50-375 Wroclaw, Poland.
Pol J Vet Sci. 2012;15(2):233-7. doi: 10.2478/v10181-011-0139-z.
Prevalence of mecA, blaZ, tetO/K/M, ermA/B/C, aph, and vanA/B/C/D genes conferring resistance to oxacillin, penicillin, tetracycline, erythromycin, gentamicin, and vancomycin was investigated in 65 staphylococcal isolates belonging to twelve species obtained from ready-to-eat porcine, bovine, and chicken products. All coagulase negative staphylococci (CNS) and S. aureus isolates harbored at least one antibiotic resistance gene. None of the S. aureus possessed more than three genes, while 25% of the CNS isolates harbored at least four genes encoding resistance to clinically used antibiotics. In 15 CNS isolates the mecA gene was detected, while all S. aureus isolates were mecA-negative. We demonstrate that in ready-to-eat food the frequency of CNS harboring multiple antibiotic resistance genes is higher than that of multiple resistant S. aureus, meaning that food can be considered a reservoir of bacteria containing genes potentially contributing to the evolution of antibiotic resistance in staphylococci.
在从即食猪、牛和鸡肉制品中分离得到的属于12个种的65株葡萄球菌中，研究了赋予对苯唑西林、青霉素、四环素、红霉素、庆大霉素和万古霉素耐药性的mecA、blaZ、tetO/K/M、ermA/B/C、aph和vanA/B/C/D基因的流行情况。所有凝固酶阴性葡萄球菌（CNS）和金黄色葡萄球菌分离株均携带至少一个抗生素耐药基因。没有一株金黄色葡萄球菌携带超过三个基因，而25%的CNS分离株携带至少四个编码对临床使用抗生素耐药性的基因。在15株CNS分离株中检测到mecA基因，而所有金黄色葡萄球菌分离株mecA均为阴性。我们证明，在即食食品中，携带多个抗生素耐药基因的CNS的频率高于多重耐药金黄色葡萄球菌，这意味着食品可被视为含有可能有助于葡萄球菌抗生素耐药性进化的基因的细菌储存库。
