Cruaud P, Yamashita J T, Casabona N M, Papa F, David H L
Unité de la Tuberculose et des Mycobactéries, Institut Pasteur, Paris.
Res Microbiol. 1990 Jul-Aug;141(6):679-94. doi: 10.1016/0923-2508(90)90062-u.
Serum IgG and IgM antibodies against a 2,3-diacyl-trehalose-2'-sulphate (SL-IV) antigen using ELISA were determined in controls (n = 288) and in leprosy (n = 210) and tuberculosis (n = 99) patients. In all assays, the amount of antigen per well was 100.0 ng and sera were diluted 1/250. In the case of leprosy, anti-SL-IV IgG and IgM antibody titres increased from the tuberculoid towards the lepromatous pole of the spectrum. In the tested population, the sensitivity of the assay was 93.2% in multibacillary leprosy and 33.3% in paucibacillary leprosy (specificity of 88.7%). Multibacillary patients with erythema nodosum leprosum (ENL) had lower titres than non-ENL. ELISA results were similar to those obtained using the Mycobacterium leprae phenolic glycolipid-I (PGL-I) antigen. In the case of tuberculosis (pulmonary and extrapulmonary), significant titres of anti SL-IV IgG and IgM antibodies were detected in about 75% of the patients using a cutoff point of 0.150, and in 51.6% using a cutoff of 0.300 (specificities were, respectively, 88% and 100%). We concluded that the determination of IgG and IgM antibodies against SL-IV was useful in leprosy and tuberculosis case finding program using a cutoff point of 0.150, and for serodiagnosis using a cutoff of 0.300.
采用酶联免疫吸附测定法（ELISA）测定了288名对照者、210名麻风病患者和99名结核病患者血清中抗2,3 - 二酰基海藻糖 - 2'- 硫酸盐（SL - IV）抗原的IgG和IgM抗体。在所有检测中，每孔抗原量为100.0 ng，血清稀释度为1/250。在麻风病患者中，抗SL - IV IgG和IgM抗体滴度从结核样型向瘤型端逐渐升高。在受试人群中，该检测方法在多菌型麻风病中的敏感性为93.2%，在少菌型麻风病中的敏感性为33.3%（特异性为88.7%）。有多形性红斑型麻风（ENL）的多菌型患者的抗体滴度低于无ENL的患者。ELISA结果与使用麻风分枝杆菌酚糖脂 - I（PGL - I）抗原获得的结果相似。在结核病（肺结核和肺外结核）患者中，使用截断值0.150时，约75%的患者检测到显著滴度的抗SL - IV IgG和IgM抗体，使用截断值0.300时，51.6%的患者检测到显著滴度的抗体（特异性分别为88%和100%）。我们得出结论，使用截断值0.150时，测定抗SL - IV的IgG和IgM抗体有助于麻风病和结核病的病例发现计划，使用截断值0.300时有助于血清学诊断。
