Faculty of Science and Technology, Department of Developmental BioEngineering, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, The Netherlands.
Tissue Eng Part B Rev. 2013 Feb;19(1):31-40. doi: 10.1089/ten.TEB.2012.0273. Epub 2012 Sep 5.
The repair of articular cartilage is challenging due to the sparse native cell population combined with the avascular and aneural nature of the tissue. In recent years, cartilage tissue engineering has shown great promise. As with all tissue engineering strategies, the possible therapeutic outcome is intimately linked with the used combination of cells, growth factors, and biomaterials. However, the optimal combination has remained a controversial topic and no consensus has been reached. In consequence, much effort has been dedicated, to further design, investigate, and optimize cartilage repair strategies. Specifically, various research groups have performed intensive investigations attempting to identify the single most optimal cell source for articular cartilage repair strategies. However, recent findings indicate that not the heavily investigated monocell source, but the less studied combinations of cell sources in coculture might be more attractive for cartilage repair strategies. This review will give a comprehensive overview on the cell sources that have been investigated for articular cartilage repair strategies. In particular, the advantages and disadvantages of investigated cell sources are comprehensively discussed with emphasis on the potential of cocultures in which benefits are combined, while the disadvantages of single-cell sources for cartilage repair are mitigated.
由于关节软骨中细胞数量稀少，且组织呈血管和神经缺乏状态，因此修复关节软骨具有挑战性。近年来，软骨组织工程展现出巨大的应用前景。与所有组织工程策略一样，可能的治疗效果与使用的细胞、生长因子和生物材料密切相关。然而，最佳组合一直是一个有争议的话题，尚未达成共识。因此，人们付出了大量努力来进一步设计、研究和优化软骨修复策略。具体来说，许多研究小组进行了深入的研究，试图确定用于关节软骨修复策略的最佳单一细胞来源。然而，最近的研究结果表明，对于软骨修复策略来说，具有吸引力的并非是经过大量研究的单细胞来源，而是在共培养中研究较少的细胞来源组合。这篇综述将全面概述已被用于关节软骨修复策略的细胞来源。特别地，全面讨论了已研究的细胞来源的优缺点，并强调了共培养的潜力，共培养中可结合优势，同时减轻单一细胞来源用于软骨修复的缺点。
