利用纳米孔单分子计数器检测 microRNA。
Detection of miRNAs with a nanopore single-molecule counter.
机构信息
Biological Engineering and Dalton Cardiovascular Research Center, University of Missouri, Columbia, MO 65211, USA.
出版信息
Expert Rev Mol Diagn. 2012 Jul;12(6):573-84. doi: 10.1586/erm.12.58.
Abstract
miRNAs are short noncoding RNA molecules that are important in regulating gene expression. Due to the correlation of their expression levels and various diseases, miRNAs are being investigated as potential biomarkers for molecular diagnostics. The fast-growing miRNA exploration demands rapid, accurate, low-cost miRNA detection technologies. This article will focus on two platforms of nanopore single-molecule approach that can quantitatively measure miRNA levels in samples from tissue and cancer patient plasma. Both nanopore methods are sensitive and specific, and do not need labeling, enzymatic reaction or amplification. In the next 5 years, the nanopore-based miRNA techniques will be improved and validated for noninvasive and early diagnosis of diseases.
摘要
miRNAs 是一类短的非编码 RNA 分子，在调节基因表达方面发挥着重要作用。由于它们的表达水平与各种疾病相关，miRNAs 被作为分子诊断的潜在生物标志物进行研究。miRNA 的快速发展需要快速、准确、低成本的 miRNA 检测技术。本文将重点介绍两种基于纳米孔单分子方法的平台，这些方法可以定量测量组织样本和癌症患者血浆中的 miRNA 水平。这两种纳米孔方法都具有高灵敏度和特异性，并且不需要标记、酶反应或扩增。在未来 5 年内，基于纳米孔的 miRNA 技术将得到改进和验证，用于疾病的非侵入性和早期诊断。
相似文献
1
Detection of miRNAs with a nanopore single-molecule counter.利用纳米孔单分子计数器检测 microRNA。
Expert Rev Mol Diagn. 2012 Jul;12(6):573-84. doi: 10.1586/erm.12.58.
2
Nanopore single-molecule detection of circulating microRNAs.循环微RNA的纳米孔单分子检测
Methods Mol Biol. 2013;1024:255-68. doi: 10.1007/978-1-62703-453-1_21.
3
Rapid electronic detection of probe-specific microRNAs using thin nanopore sensors.利用薄纳米孔传感器快速检测探针特异性 microRNAs。
Nat Nanotechnol. 2010 Nov;5(11):807-14. doi: 10.1038/nnano.2010.202. Epub 2010 Oct 24.
4
Programming nanopore ion flow for encoded multiplex microRNA detection.用于编码多重微小RNA检测的纳米孔离子流编程
ACS Nano. 2014 Apr 22;8(4):3444-50. doi: 10.1021/nn406339n. Epub 2014 Mar 26.
5
Nanopore single-molecule dielectrophoretic detection of cancer-derived microRNA biomarkers.癌症衍生微小RNA生物标志物的纳米孔单分子介电泳检测
Annu Int Conf IEEE Eng Med Biol Soc. 2013;2013:6821-4. doi: 10.1109/EMBC.2013.6611124.
6
Microfluidic primer extension assay.微流控引物延伸分析
Methods Mol Biol. 2012;822:143-52. doi: 10.1007/978-1-61779-427-8_10.
7
Nanotechnology-based strategies for the detection and quantification of microRNA.基于纳米技术的微小RNA检测与定量策略
Chemistry. 2014 Jul 28;20(31):9476-92. doi: 10.1002/chem.201402649. Epub 2014 Jul 2.
8
Single-Molecule Binding Assay Using Nanopores and Dimeric NP Conjugates.基于纳米孔和二聚体 NP 缀合物的单分子结合分析。
Adv Mater. 2021 Sep;33(38):e2103067. doi: 10.1002/adma.202103067. Epub 2021 Jul 29.
9
Polycationic Probe-Guided Nanopore Single-Molecule Counter for Selective miRNA Detection.用于选择性检测微小RNA的聚阳离子探针引导的纳米孔单分子计数器
Methods Mol Biol. 2017;1632:255-268. doi: 10.1007/978-1-4939-7138-1_17.
10
Designing a polycationic probe for simultaneous enrichment and detection of microRNAs in a nanopore.设计一种用于在纳米孔中同时富集和检测 microRNAs 的聚阳离子探针。
ACS Nano. 2013 May 28;7(5):3962-9. doi: 10.1021/nn305789z. Epub 2013 Apr 10.
引用本文的文献
1
Computational and experimental study of AC measurements performed by a double-nanohole plasmonic nanopore sensor on 20 nm silica nanoparticles.双纳米孔等离子体纳米孔传感器对20纳米二氧化硅纳米颗粒进行交流测量的计算与实验研究。
Sens Biosensing Res. 2024 Dec;46. doi: 10.1016/j.sbsr.2024.100694. Epub 2024 Sep 21.
2
Ionic Strength Investigation on the Interaction Between miR-155 and a PNA-Based Probe by Atomic Force Spectroscopy.基于原子力光谱法对miR-155与基于肽核酸的探针之间相互作用的离子强度研究
Biomolecules. 2025 Apr 28;15(5):634. doi: 10.3390/biom15050634.
3
Modelling Cancer Pathophysiology: Mechanisms and Changes in the Extracellular Matrix During Cancer Initiation and Early Tumour Growth.癌症病理生理学建模：癌症起始和早期肿瘤生长过程中细胞外基质的机制与变化
Cancers (Basel). 2025 May 15;17(10):1675. doi: 10.3390/cancers17101675.
4
Multi-physics simulations and experimental comparisons for the optical and electrical forces acting on a silica nanoparticle trapped by a double-nanohole plasmonic nanopore sensor.针对双纳米孔等离子体纳米孔传感器捕获的二氧化硅纳米颗粒上的光学力和电力进行的多物理场模拟与实验比较。
Sens Biosensing Res. 2023 Aug;41. doi: 10.1016/j.sbsr.2023.100581. Epub 2023 Aug 11.
5
An overview of recent technological developments in bovine genomics.牛基因组学近期技术发展概述。
Vet Anim Sci. 2024 Jul 23;25:100382. doi: 10.1016/j.vas.2024.100382. eCollection 2024 Sep.
6
Viral oncogenes, viruses, and cancer: a third-generation sequencing perspective on viral integration into the human genome.病毒癌基因、病毒与癌症：关于病毒整合入人类基因组的第三代测序视角
Front Oncol. 2023 Dec 21;13:1333812. doi: 10.3389/fonc.2023.1333812. eCollection 2023.
7
Therapeutic Approaches to Increase the Survival Rate of Cancer Patients in the Younger and Older Population.提高年轻和老年癌症患者生存率的治疗方法。
Curr Aging Sci. 2024;17(1):16-30. doi: 10.2174/0118746098241507231127114248.
8
Development and Characterization of Cationic Nanostructured Lipid Carriers as Drug Delivery Systems for miRNA-27a.阳离子纳米结构脂质载体作为miRNA - 27a药物递送系统的开发与表征
Pharmaceuticals (Basel). 2023 Jul 14;16(7):1007. doi: 10.3390/ph16071007.
9
Ribonucleic Acid-Mediated Control of Protein Translation Under Stress.应激状态下 RNA 介导的蛋白质翻译调控
Antioxid Redox Signal. 2023 Aug;39(4-6):374-389. doi: 10.1089/ars.2023.0233.
10
Single cell quantification of microRNA from small numbers of non-invasively sampled primary human cells.单细胞定量分析从小数目的非侵入性采样的原代人类细胞中的 microRNA。
Commun Biol. 2023 Apr 26;6(1):458. doi: 10.1038/s42003-023-04845-8.
本文引用的文献
1
MicroRNA in situ hybridization.微小RNA原位杂交
Methods Mol Biol. 2012;822:67-84. doi: 10.1007/978-1-61779-427-8_5.
2
Nanopore sensors for nucleic acid analysis.纳米孔传感器用于核酸分析。
Nat Nanotechnol. 2011 Sep 18;6(10):615-24. doi: 10.1038/nnano.2011.129.
3
Plasma microRNAs as novel biomarkers for early detection of lung cancer.血浆微小RNA作为肺癌早期检测的新型生物标志物。
Int J Clin Exp Pathol. 2011 Aug 15;4(6):575-86. Epub 2011 Aug 8.
4
Nanopore-based detection of circulating microRNAs in lung cancer patients.基于纳米孔的肺癌患者循环 microRNAs 检测。
Nat Nanotechnol. 2011 Sep 4;6(10):668-74. doi: 10.1038/nnano.2011.147.
5
RNA-ligase-dependent biases in miRNA representation in deep-sequenced small RNA cDNA libraries.深度测序小 RNA cDNA 文库中 miRNA 代表的 RNA 连接酶依赖性偏倚。
RNA. 2011 Sep;17(9):1697-712. doi: 10.1261/rna.2799511. Epub 2011 Jul 20.
6
mRNA transcript quantification in archival samples using multiplexed, color-coded probes.使用多重、彩色编码探针对存档样本进行 mRNA 转录本定量。
BMC Biotechnol. 2011 May 9;11:46. doi: 10.1186/1472-6750-11-46.
7
MicroRNA biomarkers in lung cancer: MiRacle or quagMiRe?肺癌中的 microRNA 生物标志物：Miracle 还是 Quagmire？
Transl Res. 2011 Apr;157(4):209-15. doi: 10.1016/j.trsl.2010.12.015. Epub 2011 Jan 27.
8
MicroRNA signatures in tissues and plasma predict development and prognosis of computed tomography detected lung cancer.组织和血浆中的 microRNA 特征可预测 CT 检测肺癌的发生和预后。
Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3713-8. doi: 10.1073/pnas.1100048108. Epub 2011 Feb 7.
9
Prognostic impact of MiR-155 in non-small cell lung cancer evaluated by in situ hybridization.原位杂交评估 miR-155 在非小细胞肺癌中的预后影响。
J Transl Med. 2011 Jan 10;9:6. doi: 10.1186/1479-5876-9-6.
10
miRBase: integrating microRNA annotation and deep-sequencing data.miRBase：整合微小RNA注释与深度测序数据
Nucleic Acids Res. 2011 Jan;39(Database issue):D152-7. doi: 10.1093/nar/gkq1027. Epub 2010 Oct 30.
Zotero 插件