Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK.
Rapid Commun Mass Spectrom. 2012 Sep 15;26(17):2021-6. doi: 10.1002/rcm.6311.
The recently designed dynamically harmonized Fourier transform ion cyclotron resonance (FT-ICR) cell creates a more harmonized electric field for the detection of the cyclotron motion of ions and prolongs the ion transient from seconds to minutes. In order to achieve its best performance, phase correction was applied in the spectra, and new advantages of the absorption-mode were revealed.
Spectra were acquired from both simple standard and complex mixtures using either narrowband or broadband mode, and the data were processed to compare the performance of the spectra in magnitude and absorption-mode.
The research shows that phase correction works well with data from Nikolaev's new cell, which produces the maximum improvement in resolving power (2×), and improves the match with the theoretical intensities of the isotopic peaks. In addition, the harmonic peaks can be diagnosed immediately in the absorption-mode.
The manuscript demonstrates absorption-mode spectra from Nikolaev's ICR cell, which will be of interest to the community. The improved relative peak intensities and immediate identification of harmonic peaks will facilitate data interpretation.
最近设计的动态协调傅里叶变换离子回旋共振（FT-ICR）池为检测离子回旋运动创造了更协调的电场，并将离子瞬态从秒延长到分钟。为了发挥其最佳性能，在光谱中应用了相位校正，并揭示了吸收模式的新优势。
使用窄带或宽带模式从简单的标准和复杂混合物中获取光谱，并对数据进行处理，以比较光谱在幅度和吸收模式下的性能。
研究表明，相位校正对尼古拉耶夫新池的数据效果很好，可将分辨率提高 2 倍，并提高与同位素峰理论强度的匹配度。此外，在吸收模式下可以立即诊断谐频峰。
本文展示了尼古拉耶夫 ICR 池的吸收模式光谱，这将引起科学界的兴趣。相对峰强度的提高和谐频峰的即时识别将有助于数据解释。
