Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, People's Republic of China.
Int J Nanomedicine. 2012;7:3613-24. doi: 10.2147/IJN.S32061. Epub 2012 Jul 11.
The purpose of this study was to synthesize a self-setting bioactive cement by incorporation of wollastonite nanofibers (WNFs) into calcium phosphate cement (CPC). The composition, morphology, setting time, compressive strength, hydrophilicity, and degradation of WNF-doped CPC (wnf-CPC) were investigated. Scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and inductively coupled plasma atomic emission spectroscopy were utilized. Additionally, methyl-thiazolyl-tetrazolium bromide assay, scanning electron microscopy, inductively coupled plasma atomic emission spectroscopy, and histological evaluation were used to study the cell and tissue responses to wnf-CPC, both in vitro and in vivo. The results confirmed that the addition of WNFs into CPC had no obvious effect on the setting time or the compressive strength of wnf-CPC, provided the WNF amount was not more than 10 wt%. However, the hydrophilicity and degradability of wnf-CPC were significantly improved by the addition of WNFs - this was because of the change of microstructure caused by the WNFs. The preferred dissolution of WNFs caused the formation of microporosity in wnf-CPC when soaked in tris hydrochloride solution. The microporosity enlarged the surface area of the wnf-CPC and so promoted degradation of the wnf-CPC when in contact with liquid. In addition, MG-63 cell attachment and proliferation on the wnf-CPC were superior to that on the CPC, indicating that incorporation of WNFs into CPC improved the biological properties for wnf-CPC. Following the implantation of wnf-CPC into bone defects of rabbits, histological evaluation showed that wnf-CPC enhanced the efficiency of new bone formation in comparison with CPC, indicating excellent biocompatibility and osteogenesis of wnf-CPC. In conclusion, wnf-CPC exhibited promising prospects in bone regeneration.
本研究旨在通过将硅灰石纳米纤维（WNFs）掺入磷酸钙水泥（CPC）中来合成自凝固生物活性水泥。研究了 WNF 掺杂 CPC（wnf-CPC）的组成、形态、凝固时间、抗压强度、亲水性和降解性。利用扫描电子显微镜、傅里叶变换红外光谱、X 射线衍射和电感耦合等离子体原子发射光谱进行了研究。此外，还利用噻唑蓝溴化法、扫描电子显微镜、电感耦合等离子体原子发射光谱和组织学评估研究了 wnf-CPC 在体外和体内的细胞和组织反应。结果证实，当 WNF 含量不超过 10wt%时，WNFs 对 CPC 的凝固时间或 wnf-CPC 的抗压强度没有明显影响。然而，通过添加 WNFs，wnf-CPC 的亲水性和降解性得到了显著改善-这是由于 WNFs 引起的微观结构变化所致。当在三羟盐酸溶液中浸泡时，WNF 的优先溶解导致 wnf-CPC 中形成微孔。微孔增大了 wnf-CPC 的表面积，因此在与液体接触时促进了 wnf-CPC 的降解。此外，MG-63 细胞在 wnf-CPC 上的附着和增殖优于 CPC，表明将 WNFs 掺入 CPC 提高了 wnf-CPC 的生物性能。将 wnf-CPC 植入兔骨缺损后，组织学评估表明 wnf-CPC 增强了新骨形成的效率，与 CPC 相比，表明 wnf-CPC 具有优异的生物相容性和成骨作用。总之，wnf-CPC 在骨再生方面具有广阔的前景。
