Goy Dante Pascual, Gorosito Emmanuel, Costa Hermes S, Mortarino Pablo, Pedemonte Noelia Acosta, Toledo Javier, Mansur Herman S, Pereira Marivalda M, Battaglino Ricardo, Feldman Sara
LABOATEM: Fac Cs Médicas, Universidad Nacional de Rosario, Argentina.
Open Biomed Eng J. 2012;6:85-91. doi: 10.2174/1874120701206010085. Epub 2012 Jul 10.
At present, typical approaches employed to repair fractures and other bone lesions tend to use matrix grafts to promote tissue regeneration. These grafts act as templates, which promote cellular adhesion, growth and proliferation, osteoconduction, and even osteoinduction, which commonly results in de novo osteogenesis. The present work aimed to study the bone-repairing ability of hybrid matrixes (HM) prepared with polyvinyl alcohol (PVA) and bioactive glass in an experimental rabbit model. The HM were prepared by combining 30% bioactive glass (nominal composition of 58% SiO2 -33 % CaO - 9% P2O5) and 70% PVA. New Zealand rabbits were randomly divided into the control group (C group) and two groups with bone lesions, in which one received a matrix implant HM (Implant group), while the other did not (no Implant group). Clinical monitoring showed no altered parameters from either the Implant or the no Implant groups as compared to the control group, for the variables of diet grades, day and night temperatures and hemograms. In the Implant group, radiologic and tomographic studies showed implanted areas with clean edges in femoral non-articular direction, and radio-dense images that suggest incipient integration. Minimum signs of phlogosis could be observed, whereas no signs of rejection at this imaging level could be identified. Histological analysis showed evidence of osteo-integration, with the formation of a trabecular bone within the implant. Together, these results show that implants of hybrid matrixes of bioactive glass are capable of promoting bone regeneration.
目前，用于修复骨折和其他骨损伤的典型方法倾向于使用基质移植物来促进组织再生。这些移植物充当模板，促进细胞黏附、生长和增殖、骨传导，甚至骨诱导，这通常会导致新骨形成。本研究旨在通过实验兔模型研究由聚乙烯醇（PVA）和生物活性玻璃制备的混合基质（HM）的骨修复能力。HM是通过将30%的生物活性玻璃（标称成分为58%SiO₂ - 33%CaO - 9%P₂O₅）与70%的PVA混合制备而成。将新西兰兔随机分为对照组（C组）和两组有骨损伤的组，其中一组接受基质植入物HM（植入组），另一组不接受（无植入组）。临床监测显示，与对照组相比，植入组和无植入组在饮食等级、昼夜温度和血常规等变量方面的参数没有变化。在植入组中，放射学和断层扫描研究显示，股骨非关节方向的植入区域边缘清晰，放射性致密图像表明开始融合。可以观察到最小程度的炎症迹象，而在此成像水平上未发现排斥迹象。组织学分析显示有骨整合的证据，植入物内形成了小梁骨。总之，这些结果表明生物活性玻璃混合基质植入物能够促进骨再生。
