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印度南部一家三级护理中心通过FACSCount系统对CD3 +和CD4 + T细胞进行每日质量控制。

Daily quality control in CD3+ and CD4+ T cell estimation by the FACSCount system at a tertiary care center in south India.

作者信息

Ramalingam Veena V, Mani Monika, Sundaresan Vijayanand C, Karunaiya Ramesh J, Sachithanandham Jaiprasath, Kannangai Rajesh

机构信息

Department of Clinical Virology, Christian Medical College, Vellore, India.

出版信息

Clin Vaccine Immunol. 2012 Oct;19(10):1693-6. doi: 10.1128/CVI.00246-12. Epub 2012 Aug 1.

Abstract

CD4(+) T cell count estimations are subject to high variations; hence, in this study, the previous day's tested samples were included routinely as the internal quality controls. The percentages of variation of the 2-day values were analyzed for 280 observations and the mean variation for CD4(+) and CD3(+) T cell counts ranged from 5.21% to 9.66%. This method is a good internal quality control (IQC) procedure for the estimation of CD3(+) and CD4(+) T cell counts in resource-poor settings.

摘要

CD4(+) T细胞计数的估计值存在很大差异;因此,在本研究中,前一天检测的样本被常规用作内部质量控制。对280次观察结果分析了2天数值的变化百分比,CD4(+)和CD3(+) T细胞计数的平均变化范围为5.21%至9.66%。该方法是在资源匮乏环境中估计CD3(+)和CD4(+) T细胞计数的良好内部质量控制(IQC)程序。

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本文引用的文献

1
Evaluation of a dry format reagent for CD4+ and CD8+ T-cell enumeration with FACSCount and Guava PCA [corrected].
Indian J Med Microbiol. 2011 Oct-Dec;29(4):401-5. doi: 10.4103/0255-0857.90176.

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