Central Laboratories for Frontier Technology, Kirin Holdings Company, 3377 Soutome, Sakura-city, Tochigi 329-1414, Japan. Katsunori
J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Aug 15;903:171-6. doi: 10.1016/j.jchromb.2012.07.017. Epub 2012 Jul 21.
Sulforaphane is an isothiocyanate well known for its potential health benefits. With the aim of finding sulforaphane supply sources, its precursor, glucoraphanin, was widely searched for among Brassica oleracea varieties. Quantitative profiling of seven glucosinolates by LC-MS analysis was performed on 6 cultivars of broccoli, 32 of cabbage and 24 cultivars of kale. The glucoraphanin levels found in three cultivars of cabbage and six cultivars of kale were comparable with, or even higher than, the highest of broccoli (119.4 mg/100g FW). The most promising group belonged to the black kale, Cavolo nero. Use of a C30 column and an ammonium formate buffer in LC-MS and a micro plate solid phase extraction technique was highly effective.
萝卜硫素是一种异硫氰酸酯，以其潜在的健康益处而闻名。为了寻找萝卜硫素的供应来源，人们广泛地在甘蓝属蔬菜品种中寻找其前体物质——葡萄糖苷。采用 LC-MS 分析对 6 个西兰花品种、32 个白菜品种和 24 个羽衣甘蓝品种中的 7 种硫代葡萄糖苷进行了定量分析。在 3 个白菜品种和 6 个羽衣甘蓝品种中发现的葡萄糖苷水平与西兰花（119.4mg/100g FW）的最高水平相当，甚至更高。最有前途的是黑羽衣甘蓝。在 LC-MS 中使用 C30 柱和甲酸铵缓冲液以及微孔板固相萃取技术非常有效。
