Medical Research Council Laboratory of Molecular Biology, Cambridge, UK.
Nat Chem Biol. 2012 Sep;8(9):748-50. doi: 10.1038/nchembio.1043. Epub 2012 Aug 5.
Genetic code expansion for unnatural amino acid mutagenesis has, until recently, been limited to cell culture. We demonstrate the site-specific incorporation of unnatural amino acids into proteins in Drosophila melanogaster at different developmental stages, in specific tissues and in a subset of cells within a tissue. This approach provides a foundation for probing and controlling processes in this established metazoan model organism with a new level of molecular precision.
遗传密码扩展用于非天然氨基酸诱变，直到最近，一直局限于细胞培养。我们证明了在不同的发育阶段、特定组织和组织内的细胞亚群中，非天然氨基酸在黑腹果蝇中的特异性掺入蛋白质。这种方法为在这个已建立的后生动物模式生物中以新的分子精度探测和控制过程提供了基础。
