Ghoneim Magdy A E, Moselhy Said S
1Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.
Toxicol Ind Health. 2014 Apr;30(3):225-32. doi: 10.1177/0748233712451770. Epub 2012 Aug 3.
Probiotics are known as living, nonpathogenic microorganisms that colonize the intestine and provide benefit to the host. The present study aims to measure one important energy metabolism-related enzyme activity in blood of rabbits fed on probiotics of recommended concentration. In addition, it also aims for the evaluation of the expression level of lactate dehydrogenase (LDH) enzyme using reverse transcriptase-polymerase chain reaction (RT-PCR) technique. Two groups of rabbits are used: control group receiving normal standardized diet and the other probiotic-supplemented group receiving the same diet containing probiotic, namely, Mega acidophilus (200 million cfu/kg body weight/day) for 4 weeks. The obtained results revealed that the rabbits supplemented with probiotics showed a significant decrease in the levels of serum total cholesterol (TC), triacylglycerol, high-density lipoprotein cholesterol (HDL-c) and low-density lipoprotein cholesterol (LDL-c) when compared with control group. Risk factors detected by measuring TC/HDL-c and LDL-c/HDL-c ratios showed statistically significant decrease in probiotic-supplemented rabbits when compared with control group. In addition, blood glucose and total LDH activity were elevated in probiotic-supplemented rabbits when compared with control group. RT-PCR products of LDH-M gene produced two specific amplicons. One amplicon has the expected size of 243 bp from all samples of rabbits as revealed by GelPro software. The level of LDH-M expression was found to be increased in the probiotic-supplemented group. However, unexpected amplicons are produced at 586 bp in all the samples, which may be a dimeric form of the amplified region. It was concluded that this probiotic blend is beneficiary for the metabolic reactions of lipids in the body. Moreover, LDH expression level can be considered as a biomarker for the effect of probiotic and hence monitoring the metabolic changes as reflected from its administration.
益生菌是已知的定殖于肠道并对宿主有益的活的非致病性微生物。本研究旨在测定以推荐浓度的益生菌喂养的兔子血液中一种重要的能量代谢相关酶的活性。此外，它还旨在使用逆转录聚合酶链反应（RT-PCR）技术评估乳酸脱氢酶（LDH）的表达水平。使用两组兔子：对照组接受正常标准饮食，另一组益生菌补充组接受含有益生菌即嗜酸乳杆菌（2亿cfu/千克体重/天）的相同饮食，持续4周。获得的结果显示，与对照组相比，补充益生菌的兔子血清总胆固醇（TC）、三酰甘油、高密度脂蛋白胆固醇（HDL-c）和低密度脂蛋白胆固醇（LDL-c）水平显著降低。通过测量TC/HDL-c和LDL-c/HDL-c比值检测的危险因素显示，与对照组相比，补充益生菌的兔子在统计学上有显著降低。此外，与对照组相比，补充益生菌的兔子血糖和总LDH活性升高。LDH-M基因的RT-PCR产物产生了两个特异性扩增子。如GelPro软件所示，一个扩增子在所有兔子样本中的预期大小为243bp。发现补充益生菌组中LDH-M的表达水平增加。然而，在所有样本中均在586bp处产生了意外的扩增子，这可能是扩增区域的二聚体形式。得出的结论是，这种益生菌混合物对体内脂质的代谢反应有益。此外，LDH表达水平可被视为益生菌作用的生物标志物，从而监测其给药所反映的代谢变化。
