Ghoneim Magdy A, Moselhy Said S
Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia Faculty of Veterinary Medicine, Cairo University, Giza, Egypt
Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia Faculty of Science, Ain Shams University, Cairo, Egypt.
Toxicol Ind Health. 2016 Apr;32(4):741-50. doi: 10.1177/0748233713506768. Epub 2013 Nov 20.
Excessive production of free radicals can result in tissue damage, which mainly involves generation of hydroxyl radical and other oxidants. Such free radical-induced cell damage appears to play a major role in the pathogenesis of many diseases. Probiotics have been used therapeutically to modulate immunity, improve digestive processes, lower cholesterol, treat rheumatoid arthritis, and prevent cancer. The proposed research was designed to evaluate the changes in oxidative and antioxidative profile in addition to metabolic-related hormones of living animal model, which may generally affect the health status. Two groups of rabbits (10 animals each) were allocated in hygienic cages of controlled animal house. Control group received standard diet, and the other group received the same diet containing one probiotic for 30 days. Lactate dehydrogenase (LDH) activity in leukocytes, blood glucose, reduced glutathione (GSH), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) were estimated in different tissues. Malondialdehyde (MDA) and total proteins were also determined in different tissues. Certain hormones related to metabolism and growth were also evaluated. Leukocytic LDH activity was significantly increased along with nonsignificant increase of blood glucose in probiotics-fed animals. Results showed significant decreases in the levels of triiodothyronine and thyroid-stimulating hormone but showed significant elevations in thyroxine, insulin, growth hormone, and testosterone levels in animals fed with probiotics. Total proteins content was highly significantly elevated in liver, kidneys, and muscles of probiotic-administered animals. Microsomal GSH level was significantly decreased only in skeletal muscles of probiotic-treated animals. MDA was significantly lowered in animal tissues fed with probiotics. GSH-Px activity was elevated in hepatic and muscular microsomes of probiotic-supplemented animals while it was nonsignificantly increased in renal microsomes. Microsomal SOD activity was elevated in liver, kidneys, and skeletal muscles of probiotics-administrated animals. It is concluded that supplementation of probiotic may enhance antioxidant efficacy and scavenge free radicals and thus may be used as a preventive measure for protection against free radicals-induced disorders.
自由基的过量产生会导致组织损伤，这主要涉及羟自由基和其他氧化剂的生成。这种自由基诱导的细胞损伤似乎在许多疾病的发病机制中起主要作用。益生菌已被用于治疗性调节免疫、改善消化过程、降低胆固醇、治疗类风湿性关节炎和预防癌症。拟进行的研究旨在评估活体动物模型中除代谢相关激素外的氧化和抗氧化状态的变化，这些变化可能会普遍影响健康状况。将两组兔子（每组10只）安置在可控动物房的卫生笼中。对照组给予标准饮食，另一组给予含一种益生菌的相同饮食，持续30天。对不同组织中的白细胞乳酸脱氢酶（LDH）活性、血糖、还原型谷胱甘肽（GSH）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）进行了评估。还测定了不同组织中的丙二醛（MDA）和总蛋白。还评估了某些与代谢和生长相关的激素。喂食益生菌的动物白细胞LDH活性显著增加，血糖无显著增加。结果显示，喂食益生菌的动物三碘甲状腺原氨酸和促甲状腺激素水平显著降低，但甲状腺素、胰岛素、生长激素和睾酮水平显著升高。益生菌给药动物的肝脏、肾脏和肌肉中总蛋白含量显著升高。仅在益生菌处理动物的骨骼肌中微粒体GSH水平显著降低。喂食益生菌的动物组织中MDA显著降低。补充益生菌的动物肝脏和肌肉微粒体中GSH-Px活性升高，而肾脏微粒体中无显著增加。益生菌给药动物肝脏、肾脏和骨骼肌中微粒体SOD活性升高。结论是，补充益生菌可能增强抗氧化功效并清除自由基，因此可作为预防自由基诱导疾病的保护措施。
