College of Food Science and Engineering, Shandong Agricultural University, Taian 271018, PR China.
Food Chem. 2012 Nov 15;135(2):332-7. doi: 10.1016/j.foodchem.2012.04.145. Epub 2012 May 11.
Hexahydrocurcumin, 1-dehydro-[6]-gingerdione, 6-dehydroshogaol and 6-shogaol were evaluated for their antioxidant and anti-inflammatory activities in the present study. The relative antioxidant potencies of ginger compounds decreased in similar order of 1-dehydro-[6]-gingerdione, hexahydrocurcumin>6-shogaol>6-dehydroshogaol in both 1,1-diphenyl-2-picyrlhydrazyl (DPPH) radical-scavenging and trolox equivalent antioxidant capacity (TEAC) assays. All tested compounds could attenuate lipopolysaccharide (LPS)-elicited increase of prostaglandin E2 (PGE(2)) in murine macrophages (RAW 264.7) in a concentration-dependent manner but hexahydrocurcumin of 7μM and 6-shogaol of 7μM. The strongest inhibitory effect was observed for 6-dehydroshogaol and 6-shogaol at 14μM with the inhibition of 53.3% and 48.9%, respectively. Furthermore, both 6-dehydroshogaol and 1-dehydro-[6]-gingerdione significantly suppressed the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) proteins in a concentration-dependent fashion. These results contribute to our theoretical understanding of the potential beneficial effects of consuming ginger as a food and/or dietary supplement.
在本研究中，评估了六氢姜黄素、1-脱氢-[6]-姜二酮、6-脱氢姜烯酮和 6-姜烯酚的抗氧化和抗炎活性。在 1,1-二苯基-2-苦基肼（DPPH）自由基清除和 Trolox 等效抗氧化能力（TEAC）测定中，姜黄素化合物的相对抗氧化能力按 1-脱氢-[6]-姜二酮、六氢姜黄素>6-姜烯酚>6-脱氢姜烯酮的相似顺序降低。所有测试的化合物都可以在浓度依赖性方式下减轻脂多糖（LPS）诱导的小鼠巨噬细胞（RAW 264.7）前列腺素 E2（PGE（2））的增加，但 7μM 的六氢姜黄素和 7μM 的 6-姜烯酚除外。在 14μM 时，6-脱氢姜烯酮和 6-姜烯酚的抑制作用最强，抑制率分别为 53.3%和 48.9%。此外，6-脱氢姜烯酮和 1-脱氢-[6]-姜二酮均能以浓度依赖性方式显著抑制诱导型一氧化氮合酶（iNOS）和环氧化酶-2（COX-2）蛋白的表达。这些结果有助于我们从理论上理解食用生姜作为食品和/或膳食补充剂的潜在有益效果。
