Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. da República, 2780-157 Oeiras, Portugal.
Food Chem. 2012 Nov 15;135(2):562-70. doi: 10.1016/j.foodchem.2012.05.023. Epub 2012 May 11.
The increase in population lifespan has enhanced the incidence of neurodegenerative diseases, for which there is, as yet, no cure. We aimed to chemically characterize phenolic-enriched fractions (PEFs) from four wild Juniperus sp. found in Portugal (Juniperus navicularis, Juniperus oxycedrus badia, Juniperus phoenicea and Juniperus turbinata) and address their potential as sources of natural products for treatment of neurodegenerative diseases. Leaves from the four Juniperus sp. evaluated contained a range of phenolic components which differed quantitatively between the species. The PEFs obtained were rich sources of phenolic compounds, exhibited acetylcholinesterase (AChE) inhibitory activity and also displayed effective intracellular radical scavenging properties in neurons submitted to oxidative injury but showed a different order of effectiveness compared to AChE inhibition. These properties made them good candidates for testing in a neurodegeneration cell model. Pre-incubation with J. oxycedrus badia PEF for 24h protected neurons from injury in the neurodegeneration cell model.
人口寿命的增加增加了神经退行性疾病的发病率，而目前这种疾病尚无治愈方法。我们的目的是对来自葡萄牙的四种野生 Juniperus sp.（Juniperus navicularis、Juniperus oxycedrus badia、Juniperus phoenicea 和 Juniperus turbinata）中富含酚类的部分（PEFs）进行化学表征，并探讨它们作为治疗神经退行性疾病天然产物的潜在用途。评估的四种 Juniperus sp. 的叶子中含有一系列酚类成分，其在不同种间存在定量差异。获得的 PEFs 是酚类化合物的丰富来源，表现出乙酰胆碱酯酶（AChE）抑制活性，并且在神经元受到氧化损伤时也表现出有效的细胞内自由基清除特性，但与 AChE 抑制相比，其有效性的顺序不同。这些特性使它们成为在神经退行性变细胞模型中进行测试的良好候选物。用 J. oxycedrus badia PEF 预孵育 24 小时可保护神经元免受神经退行性变细胞模型中的损伤。
