Microbiology Program, Department of Biological Sciences, Faculty of Science, Kuwait University, PO Box 5969, 13060 Safat, Kuwait.
Extremophiles. 2012 Sep;16(5):751-8. doi: 10.1007/s00792-012-0471-y. Epub 2012 Aug 7.
Hypersaline soil and pond water samples were mixed with 3 % crude oil, some samples were autoclaved to serve as sterile controls; experimental samples were not sterilized. After 6-week incubation at 40 °C under light/dark cycles, the soil microflora consumed 66 %, and after 4 weeks the pond water microflora consumed 63 % of the crude oil. Soil samples treated with 3 % casaminoacids lost 89 % of their oil after 6 weeks and water samples lost 86 % after 4 weeks. Samples treated with casaminoacids and antibiotics that selectively inhibited bacteria, lost even more oil, up to 94 %. Soil-water mixtures incubated under continuous illumination lost double as much more oil than samples incubated in the dark. The soil-water mixture at time zero contained 1.3 × 10(4) CFU g(-1) of hydrocarbon-utilizing microorganisms which were affiliated to Halomonas aquamarina, Exiguobacterium aurantiacum, Haloferax sp., Salinococcus sp., Marinococcus sp. and Halomonas sp. After 6-week incubation with oil, these numbers were 8.7 × 10(7) CFU g(-1) and the Haloferax sp. proportion in the total microflora increased from 20 to 93 %. Experiments using the individual cultures and three other haloarchaea isolated earlier from the same site confirmed that casaminoacids and light enhanced their oil consumption potential in batch cultures.
高盐土壤和池塘水样与 3%的原油混合，部分样品进行高压灭菌作为无菌对照；实验样品未进行灭菌。在 40°C 光照/黑暗循环下孵育 6 周后，土壤微生物消耗了 66%的原油，而在 4 周后池塘水微生物消耗了 63%的原油。用 3%的水解酪蛋白处理的土壤样品在 6 周后损失了 89%的石油，而水样在 4 周后损失了 86%。用水解酪蛋白和选择性抑制细菌的抗生素处理的样品损失了更多的石油，高达 94%。在连续光照下孵育的土壤-水混合物比在黑暗中孵育的样品损失了两倍多的石油。零时刻的土壤-水混合物含有 1.3×10(4) CFU g(-1)的烃类利用微生物，它们与海盐水生菌、橙色极端杆菌、盐单胞菌、盐球菌、海洋球菌和海盐水生菌有关。在与油孵育 6 周后，这些数量增加到 8.7×10(7) CFU g(-1)，并且盐单胞菌在总微生物群中的比例从 20%增加到 93%。使用个体培养物和从同一地点早些时候分离出的另外三种盐杆菌进行的实验证实，水解酪蛋白和光照增强了它们在批式培养中的石油消耗潜力。
