Department of Biological Sciences, Faculty of Science, Kuwait University, PO Box 5969, Safat, 13060, Kuwait City, Kuwait.
Environ Sci Pollut Res Int. 2014 Mar;21(5):3386-94. doi: 10.1007/s11356-013-2293-6. Epub 2013 Nov 16.
There is no research published sofar on managements that could bioremediate hypersaline soils and water polluted with hydrocarbons. The objective of this study was to assess the effect of vitamin amendment on hydrocarbon removal by microorganisms indigenous to such hypersaline environments. We used in this study ten hydrocarbonoclastic bacterial species and five archaeal species that had been isolated by the conventional plating method on media containing oil as a sole carbon source, from a hypersaline (3-4 M NaCl) coastal area in Kuwait, and characterized by sequencing of their 16S rRNA coding genes. The oil and pure hydrocarbon consumption was measured by gas-liquid chromatography. The oil and pure hydrocarbon consumption potential of all microorganisms in media with hypersalinity was enhanced by vitamin fertilization. This was true for individual microorganisms in pure cultures as well as for microbial consortia in hypersaline soil and water samples used as inocula. Most effective vitamins were thiamin, pyridoxine and vitamin B12. Vitamin fertilization using vitamin rich wastes or byproducts could be an effective practice for enhancing bioremediation of oil contaminated hypersaline environments.
目前尚无研究报道针对可生物修复高盐土壤和受碳氢化合物污染的水的管理方法。本研究的目的是评估维生素添加对源自这种高盐环境的微生物去除碳氢化合物的效果。我们使用了十种烃类降解细菌和五种古细菌，这些细菌是通过常规平板法在含有油作为唯一碳源的培养基上从科威特沿海地区（盐度为 3-4 M NaCl）分离出来的，并通过测序其 16S rRNA 编码基因进行了表征。通过气液色谱法测量油和纯碳氢化合物的消耗。在高盐度培养基中，维生素的添加增强了所有微生物对油和纯碳氢化合物的消耗能力。这对于纯培养中的单个微生物以及用作接种物的高盐土壤和水样中的微生物群落都是如此。最有效的维生素是硫胺素、吡哆醇和维生素 B12。利用富含维生素的废物或副产品进行维生素施肥可能是增强受油污染的高盐环境生物修复的有效方法。
