Department of Biological Sciences, Faculty of Science, Kuwait University, Kuwait.
Can J Microbiol. 2013 Dec;59(12):837-44. doi: 10.1139/cjm-2013-0698. Epub 2013 Nov 7.
Ten hydrocarbonoclastic halobacterial species and 5 haloarchaeal species that had been isolated on a mineral medium with oil as the sole carbon source grew better and consumed more crude oil, as measured by gas-liquid chromatography, in media receiving between 0.50 and 0.75 mol/L KCl and between 1.50 and 2.25 mol/L MgSO₄. Chemical analysis revealed that within a certain limit, the higher the KCl and MgSO₄ concentrations in the medium, the more K⁺ and Mg²⁺, respectively, was accumulated by cells of all the tested halobacteria and haloarchaea. Also, in experiments in which total natural microbial consortia in hypersaline soil and water samples were directly used as inocula, the consumption of hydrocarbons was enhanced in the presence of the above given concentrations of KCl and MgSO₄. It was concluded that amendment with calculated concentrations of K⁺ and Mg²⁺ could be a promising practice for hydrocarbon bioremediation in hypersaline environments.
已从以油为唯一碳源的矿物培养基中分离出 10 种烃类嗜盐菌和 5 种嗜盐古菌，它们在含有 0.50-0.75mol/L KCl 和 1.50-2.25mol/L MgSO₄的培养基中生长得更好，消耗的原油量更多，这一点可通过气液色谱法来衡量。化学分析表明，在一定范围内，培养基中 KCl 和 MgSO₄的浓度越高，所有测试的嗜盐菌和嗜盐古菌细胞分别积累的 K⁺和 Mg²⁺就越多。此外，在直接使用高盐土壤和水样中天然微生物群落作为接种物的实验中，在上述给定浓度的 KCl 和 MgSO₄存在的情况下，烃类的消耗得到了增强。结论认为，用计算浓度的 K⁺和 Mg²⁺进行改良可能是高盐环境中烃类生物修复的一种很有前景的做法。
