Oulé Mathias K, Azinwi Richard, Bernier Anne-Marie, Kablan Tano, Maupertuis Anne-Marie, Mauler Stephanie, Nevry Rose K, Dembélé Korami, Forbes Lorraine, Diop Lamine
Department of Biological Science, Faculty of Science, Collège Universitaire de Saint-Boniface, 200 Avenue de la Cathédrale, Winnipeg, Manitoba R2H 0H7, Canada.
Unité de Formation et de Recherche en Sciences et Technologie des Aliments, Abobo Adjamé University, 02 BP 801, Abidjan 02, Cote d'Ivoire.
J Med Microbiol. 2008 Dec;57(Pt 12):1523-1528. doi: 10.1099/jmm.0.2008/003350-0.
Polyhexamethylene guanidine hydrochloride (PHMGH), an antimicrobial biocide of the guanidine family, was tested for efficacy against quality-control strains of Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella choleraesuis, meticillin-resistant S. aureus (MRSA) and Escherichia coli. Bactericidal activity against S. aureus, P. aeruginosa and Salmonella choleraesuis was determined using the official methods of analysis of the Association of Official Analytical Chemists, with modifications as recommended by the Canadian General Standards Board. For MRSA and E. coli, the MIC and minimal bactericidal concentration were determined using the broth dilution technique. The experiments were carried out at 20 degrees C under a range of conditions including varying PHMGH concentration (0.001-0.1 %), contact time (0.5-10 min) and water type (distilled, tap and hard water). The phenol coefficient values determined with S. aureus, Salmonella choleraesuis and P. aeruginosa were 7.5, 6.1 and 5, respectively. No matter what type of water was used to make the dilutions, PHMGH killed MRSA and E. coli at concentrations as low as 0.04 and 0.005 % (w/v), respectively, within 1.5 min. The mode of action of PHMGH was elucidated by transmission electron microscopy: the cell envelope was broken, resulting in cell content leakage into the medium. The ultimate aim of this study was to show that PHMGH can be used as an odourless, colourless, non-corrosive and harmless disinfectant for hospital and household facilities.
聚六亚甲基胍盐酸盐（PHMGH）是胍类家族的一种抗菌杀菌剂，针对金黄色葡萄球菌、铜绿假单胞菌、猪霍乱沙门氏菌、耐甲氧西林金黄色葡萄球菌（MRSA）和大肠杆菌的质控菌株进行了功效测试。采用美国官方分析化学家协会的官方分析方法，并根据加拿大通用标准委员会的建议进行修改，测定了对金黄色葡萄球菌、铜绿假单胞菌和猪霍乱沙门氏菌的杀菌活性。对于MRSA和大肠杆菌，使用肉汤稀释技术测定最低抑菌浓度（MIC）和最低杀菌浓度。实验在20摄氏度下进行，条件包括不同的PHMGH浓度（0.001 - 0.1%）、接触时间（0.5 - 10分钟）和水的类型（蒸馏水、自来水和硬水）。用金黄色葡萄球菌、猪霍乱沙门氏菌和铜绿假单胞菌测定的苯酚系数值分别为7.5、6.1和5。无论使用何种类型的水进行稀释，PHMGH在浓度低至0.04%（w/v）和0.005%（w/v）时，分别在1.5分钟内杀死MRSA和大肠杆菌。通过透射电子显微镜阐明了PHMGH的作用方式：细胞包膜破裂，导致细胞内容物泄漏到培养基中。本研究的最终目的是表明PHMGH可作为医院和家庭设施中无味、无色、无腐蚀性且无害的消毒剂。
