Bielecki Tomasz, Cieslik-Bielecka Agata, Zelawski Maciej, Mikusek Wojciech
Department and Clinic of Orthopaedics and Trauma Surgery, Medical University of Silesia, Sosnowiec, Poland.
Transfus Apher Sci. 2012 Oct;47(2):133-8. doi: 10.1016/j.transci.2012.06.017. Epub 2012 Aug 4.
During platelet activation over 30 growth factors are released from their alpha granules. By concentrating platelets, higher levels of growth factors are obtained and can be used to stimulate the healing processes. Moreover, during blood centrifugation, huge numbers of leukocytes and thrombocytes can be obtained to form leukocyte and platelet-rich plasma (L-PRP). After platelet degranulation the active gelatinous matrix called leukocyte and platelet-rich gel (L-PRG) is formed. In the present clinical study, we report the influence of PLRG and freeze-dried allografts on healing of large femoral bone cysts. Each of 6 participants was followed on a regular basis with clinical examinations, roentgenograms and dual-energy X-ray absorptiometry (DXA) examinations. Our four-year follow up clinical study using allografts and PLRG in the treatment of large cystic lesions of the proximal femur showed that mixing these substances is not efficient and it might induce unknown local reactions between them causing complete bone graft destruction. Further studies are necessary to fully understand the mechanisms of the local activity of L-PRP gel.
在血小板活化过程中，超过30种生长因子从其α颗粒中释放出来。通过浓缩血小板，可以获得更高水平的生长因子，并可用于刺激愈合过程。此外，在血液离心过程中，可以获得大量白细胞和血小板，以形成富含白细胞和血小板的血浆（L-PRP）。血小板脱颗粒后，形成称为富含白细胞和血小板的凝胶（L-PRG）的活性凝胶状基质。在本临床研究中，我们报告了L-PRG和冻干同种异体移植物对大型股骨囊肿愈合的影响。对6名参与者中的每一位进行定期的临床检查、X线片和双能X线吸收测定（DXA）检查。我们使用同种异体移植物和L-PRG治疗股骨近端大型囊性病变的四年随访临床研究表明，混合这些物质并不有效，并且可能会在它们之间引发未知的局部反应，导致骨移植完全破坏。需要进一步研究以充分了解L-PRP凝胶局部活性的机制。
