Department of Nutritional Sciences, Emerging Field Oxidative Stress and DNA Stability, University of Vienna, Austria.
Mutagenesis. 2012 Nov;27(6):731-5. doi: 10.1093/mutage/ges039. Epub 2012 Aug 8.
Circulating unconjugated bilirubin (UCB) has been reported to protect against lung and colorectal cancer. The present study aimed to explore, for the first time, whether mildly elevated circulating UCB, as found in Gilbert`s syndrome (GS), is associated with changes of DNA damage. A random 76 individuals, matched for age and gender, were recruited from the general population and allocated into the GS group (UCB ≥ 17.1 µM; n = 38) or control group (UCB <17.1 µM; n = 38). Chromosomal and cytological changes were determined in lymphocytes and buccal cells using the cytokinesis-block micronucleus cytome assay (CBMN) and buccal micronucleus cytome assay (BMcyt). No significant differences were found between GS subjects and the control group in the CBMN and BMcyt determined endpoints. Subsequently, when age dependency of effects were analysed, lower formation of buccal micronucleated cells (by 73.3%) and buccal nuclear buds (by 70.9%) in the GS subgroup ≥ 30 years were found, compared to the GS subgroup <30 years. These findings suggest DNA protection in epithelial tissue of older individuals with GS.
循环未结合胆红素（UCB）已被报道可预防肺癌和结直肠癌。本研究旨在首次探讨吉尔伯特综合征（GS）中发现的轻度升高的循环 UCB 是否与 DNA 损伤的变化有关。随机招募了 76 名年龄和性别相匹配的普通人群，并将其分为 GS 组（UCB≥17.1µM；n=38）和对照组（UCB<17.1µM；n=38）。使用胞质分裂阻断微核细胞微核检测（CBMN）和口腔微核细胞微核检测（BMcyt）在淋巴细胞和口腔细胞中测定染色体和细胞学变化。在 CBMN 和 BMcyt 测定的终点方面，GS 受试者与对照组之间未发现显著差异。随后，当分析年龄依赖性效应时，发现年龄≥30 岁的 GS 亚组中口腔微核细胞形成减少（减少 73.3%）和口腔核芽形成减少（减少 70.9%），而年龄<30 岁的 GS 亚组则没有。这些发现表明，GS 老年个体的上皮组织中存在 DNA 保护作用。
