Department of Applied Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland.
Int J Food Microbiol. 2012 Sep 3;158(3):203-8. doi: 10.1016/j.ijfoodmicro.2012.07.016. Epub 2012 Jul 22.
A total of 471 Listeria monocytogenes isolates from different types of food and food-related sources in Poland during 2004-2010 were examined. This number includes 200 isolates from fish, 144 from fresh and frozen vegetables, 43 ready-to-eat products (deli foods, cold cuts), 13 from dairy products, 16 from raw meats, 15 from confectionery products and 40 directly from processing plants. All isolates were subjected to serotyping and lineage assays using PCR, and antimicrobial susceptibility using E-test and a broth microdilution method. Of all isolates, 256 (54.4%), 120 (25.5%), 59 (12.5%), 36 (7.6%) were identified as serotypes 1/2a (or 3a), 1/2c (or 3c), 1/2b (or 3b or 7), and 4b (or 4d or 4e), respectively. A direct correlation between the most common serotypes and three L. monocytogenes lineages was also observed. All L. monocytogenes isolates belonged to lineages I (20.2%) and II (79.8%). All strains were sensitive to ampicillin, amoxicillin, gentamicin, erythromycin, trimethoprim, rifampicin, vancomycin, chloramphenicol and sulfamethoxazol. Two of the L. monocytogenes strains (0.42%) showed phenotypic resistance. One strain was resistant to tetracycline and minocycline due to the presence of tet(M). It did not carry gene int, which may indicate that the tet(M) gene in this strain was not integrated in the transposon Tn916-Tn1545 family. The resistance of the second strain to ciprofloxacin and norfloxacin was attributed to active efflux associated with overexpression of gene lde. Our data indicate the low prevalence of antimicrobial resistance among L. monocytogenes isolates from food and food-related sources in Poland.
对 2004-2010 年期间波兰不同类型食品和食品相关来源的 471 株李斯特菌分离株进行了研究。这包括 200 株来自鱼类，144 株来自新鲜和冷冻蔬菜，43 株即食产品（熟食、冷切），13 株来自乳制品，16 株来自生肉，15 株来自糖果产品，40 株直接来自加工厂。所有分离株均进行血清分型和谱系检测，采用 PCR 技术，药敏试验采用 E-试验和肉汤微量稀释法。所有分离株中，256 株（54.4%）、120 株（25.5%）、59 株（12.5%）、36 株（7.6%）分别鉴定为 1/2a（或 3a）、1/2c（或 3c）、1/2b（或 3b 或 7）和 4b（或 4d 或 4e）血清型。还观察到最常见血清型与三种李斯特菌谱系之间存在直接相关性。所有李斯特菌分离株均属于 I 谱系（20.2%）和 II 谱系（79.8%）。所有菌株均对氨苄西林、阿莫西林、庆大霉素、红霉素、甲氧苄啶、利福平、万古霉素、氯霉素和磺胺甲恶唑敏感。有两株李斯特菌（0.42%）表现出表型耐药性。一株因携带 tet(M)而对四环素和米诺环素耐药。该菌株不携带 int 基因，这可能表明该菌株中的 tet(M)基因未整合到转座子 Tn916-Tn1545 家族中。第二株对环丙沙星和诺氟沙星的耐药性归因于基因 lde 的过度表达引起的主动外排。我们的数据表明，波兰食品和食品相关来源的李斯特菌分离株对抗生素的耐药率较低。
